Surface Plasmon Resonance Immunosensor for IgE Analysis Using Two Types of Anti-IgE Antibodies with Different Active Recognition Sites
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- LI Yan
- Department of Applied Chemistry, Graduate School of Engineering, Kyushu University
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- REN Jujie
- Department of Applied Chemistry, Graduate School of Engineering, Kyushu University
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- NAKAJIMA Hizuru
- Department of Applied Chemistry, Graduate School of Engineering, Kyushu University
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- SOH Nobuaki
- Department of Applied Chemistry, Graduate School of Engineering, Kyushu University
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- NAKANO Koji
- Department of Applied Chemistry, Graduate School of Engineering, Kyushu University
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- IMATO Toshihiko
- Department of Applied Chemistry, Graduate School of Engineering, Kyushu University
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抄録
A simple and novel method for the determination of an IgE antibody based on a surface plasmon resonance immunosensor for the diagnosis of an allergy is described. The method involves the use of an anti-IgE(D) antibody and an anti-IgE(H) antibody, which reacts with the Ce2 domain and the Ce3 domain of the IgE antibody. The anti-IgE(D) antibody was immobilized on the gold surface of a sensor chip by physical adsorption. An IgE antibody sample was incubated by adding it to an anti-IgE(H) antibody solution to form an anti-IgE(H) immunocomplex through a reaction of the Ce3 domain of the IgE antibody. The incubated solution was introduced onto the sensor chip and the immunocomplex of the IgE-anti-IgE(H) then reacted with the anti-IgE(D) antibody immobilized on the sensor chip through the Ce2 domain of the IgE antibody part of the IgE-anti-IgE(H) immunocomplex. The detection limit of the present method for the determination of the IgE antibody was about 10 ppb. The affinity constants for the anti-IgE(H) antibody immunocomplex with the IgE antibody in solution and that of the anti-IgE(H) antibody immunocomplex with the IgE antibody immobilized on the sensor chip by a biotin-streptavidin interaction were estimated to be 4.1 × 107 M-1 and 5.8 × 106 M-1, respectively. The affinity constant for the immunocomplex of the anti-IgE(H) antibody with the IgE antibody with the anti-IgE(D) immobilized on the sensor chip was estimated to be 4.9 × 107 M-1, 20-times larger than the affinity constant for the IgE antibody immunocomplex with the anti-IgE(D) antibody immobilized on the sensor chip, based on a direct immunoassay method of the IgE antibody under the same experimental conditions.
収録刊行物
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- Analytical Sciences
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Analytical Sciences 23 (1), 31-38, 2007
社団法人 日本分析化学会
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詳細情報 詳細情報について
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- CRID
- 1390001204255348480
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- NII論文ID
- 130004441364
- 10018464003
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- NII書誌ID
- AA10500785
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- COI
- 1:STN:280:DC%2BD2s%2Fhs1Kjtw%3D%3D
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- ISSN
- 13482246
- 09106340
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- NDL書誌ID
- 8598069
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- Web Site
- http://id.ndl.go.jp/bib/8598069
- https://ndlsearch.ndl.go.jp/books/R000000004-I8598069
- https://link.springer.com/content/pdf/10.2116/analsci.23.31.pdf
- https://link.springer.com/article/10.2116/analsci.23.31/fulltext.html
- http://www.jstage.jst.go.jp/article/analsci/23/1/23_1_31/_pdf
- https://search.jamas.or.jp/link/ui/2007175372
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- en
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