Enzyme-Linked Competitive Binding Assays Based on the Biotin/Avidin Interaction.
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- CHO Ho Choll
- Department of Chemistry, Kwangwoon University
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- LEE Dong Joo
- Department of Chemistry, Kwangwoon University
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- KIM So Young
- Department of Chemistry, Seoul Women’s University
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- KIM Jong-Hyun
- Department of Chemistry, Seoul Women’s University
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- PAENG Insook Rhee
- Department of Chemistry, Seoul Women’s University
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- CHA Geun Sig
- Department of Chemistry, Kwangwoon University
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Abstract
The strong interaction between biotin and avidin is utilized for the development of enzyme-linked competitive binding assays not only for biotin itself, but also for other biomolecules. Alkaline phosphatase, a single substrate enzyme typically used for heterogeneous types of competitive binding assays, is employed also for homogeneous types. For the biotin assay, the heterogeneous protocol offers a much improved detection capability when compared to the homogeneous type. A simple approach of simulating dose-response curves is introduced. An effective analyte concentration attached to an enzyme label is determined by using the same binder, but with a different enzyme label. The analyte system adapted to the biotin/avidin-mediated homogeneous protocol is digoxin and a monoclonal anti-digoxin antibody. The detection capabilities of these assays, particularly the homogeneous types, are shown to be limited by the detectability of the enzyme conjugate employed.
Journal
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- Analytical Sciences
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Analytical Sciences 15 (4), 343-347, 1999
The Japan Society for Analytical Chemistry
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Details 詳細情報について
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- CRID
- 1390001204255364352
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- NII Article ID
- 10002419361
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- NII Book ID
- AA10500785
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- COI
- 1:CAS:528:DyaK1MXisVSntLw%3D
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- ISSN
- 13482246
- 09106340
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- NDL BIB ID
- 4712399
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed