Ferritin Immunosensing on Microfabricated Electrodes Based on the Integration of Immunoprecipitation and Electrochemical Signaling Reactions
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- YOON Hyun C.
- Department of Biotechnology, Ajou University
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- YANG Haesik
- Electronics and Telecommunications Research Institute
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- BYUN Sang Yo
- Department of Biotechnology, Ajou University
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A signal registration strategy from micropatterned immunosensors that converts antigen-antibody binding reactions into electrochemical signals was demonstrated. An array-type micropatterned gold electrode on a silicon wafer was fabricated, containing two electrode geometries of rectangular (100 µm × 500 µm) and circular (r. 50 µm) types, exhibiting electrochemical characteristics of bulk and micro-electrodes, respectively. Ferritin was employed as a model analyte for immunosensing because it has an advantageous molecular structure for functionalization to the sensing interface, and is regarded as a general marker protein for tumors and cancer recurrence. With the fabricated and ferritin-functionalized immunosensors, biospecific interactions were performed with antiferritin antiserum and secondary antibody samples, followed by electrochemical signaling via an immunoprecipitation reaction by the label enzyme. Under the optimized affinity-surface construction steps and reaction conditions, both types of microfabricated electrodes exhibited well-defined calibration results as a function of the protein concentration in antiserum samples. Furthermore, circular-type micropatterned immunoelectrodes exhibited voltammetric characteristics of microelectrodes, which is advantageous in terms of sensor operation under a fixed potential and low signal drift during the signaling reaction compared with the bulk-type electrodes. The results support that the employed signaling method with the proposed immunosensor configuration is fit for sensor miniaturization and integration to future biomicrosystems.
収録刊行物
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- Analytical Sciences
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Analytical Sciences 20 (9), 1249-1253, 2004
社団法人 日本分析化学会
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詳細情報 詳細情報について
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- CRID
- 1390001204255452032
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- NII論文ID
- 130004440982
- 10013524270
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- NII書誌ID
- AA10500785
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- COI
- 1:CAS:528:DC%2BD2cXnvVGru70%3D
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- ISSN
- 13482246
- 09106340
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- NDL書誌ID
- 7078584
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- PubMed
- 15478331
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- Web Site
- http://id.ndl.go.jp/bib/7078584
- https://ndlsearch.ndl.go.jp/books/R000000004-I7078584
- https://link.springer.com/content/pdf/10.2116/analsci.20.1249.pdf
- https://link.springer.com/article/10.2116/analsci.20.1249/fulltext.html
- http://www.jstage.jst.go.jp/article/analsci/20/9/20_9_1249/_pdf
- https://search.jamas.or.jp/link/ui/2006059911
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
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- PubMed
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