Piezo Electric Sensor for Endocrine-Disrupting Chemicals Using Receptor-co-factor Interaction

MURATA Masaharu, GOUDA Chifumi, YANO Kentaro, KUROKI Shinichiro, SUZUTANI Tatsuo, KATAYAMA Yoshiki

doi:10.2116/analsci.19.1355

Piezo Electric Sensor for Endocrine-Disrupting Chemicals Using Receptor-co-factor Interaction

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MURATA Masaharu

Department of Applied Chemistry, Graduate School of Engineering, Kyushu University
GOUDA Chifumi

Department of Applied Chemistry, Graduate School of Engineering, Kyushu University
YANO Kentaro

Department of Applied Chemistry, Graduate School of Engineering, Kyushu University
KUROKI Shinichiro

Department of Applied Chemistry, Graduate School of Engineering, Kyushu University
SUZUTANI Tatsuo

Department of Microbiology, School of Medicine, Fukushima Medical University
KATAYAMA Yoshiki

Department of Applied Chemistry, Graduate School of Engineering, Kyushu University

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抄録

In vitro screening assays are useful techniques for the determination of receptor-mediated activities in environmental samples. In order to define whether environmental chemicals act as an agonist or antagonist to the human estrogen receptor (hER), we have constructed a biosensor based on ligand-inducible interactions between hER and relative proteins on a quartz crystal microbalance (QCM). The his-tagged proteins, which were expressed in E. coli by recombinant DNA technology, were immobilized on an Au-electrode with Ni(II)-mediated chemisorption using the histidine tag and thiol-modified iminodiacetic acid. The resonance-frequency change of the protein-modified electrode was caused by association or dissociation with the hER relative proteins on the surface in the presence of estrogen. These results suggest that this sensor is applicable as a large-scale screening tool for estrogenic compounds.