Immunoassay Using Chemiluminescence Detection of Dyestuff-Containing Liposomes as a Labeling Reagent.

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  • TSUKAGOSHI Kazuhiko
    Department of Chemical Engineering and Materials Science, Faculty of Engineering, Doshisha University
  • AKASAKA Hideshi
    Department of Chemical Engineering and Materials Science, Faculty of Engineering, Doshisha University
  • OKUMURA Yasuo
    Department of Chemical Engineering and Materials Science, Faculty of Engineering, Doshisha University
  • FUKAYA Ryosuke
    Department of Chemical Engineering and Materials Science, Faculty of Engineering, Doshisha University
  • OTSUKA Miwa
    Department of Chemical Engineering and Materials Science, Faculty of Engineering, Doshisha University
  • FUJIWARA Kazutoshi
    Department of Chemical Engineering and Materials Science, Faculty of Engineering, Doshisha University
  • UMEHARA Hiroyuki
    Department of Chemical Engineering and Materials Science, Faculty of Engineering, Doshisha University
  • MAEDA Reiko
    Department of Chemical Engineering and Materials Science, Faculty of Engineering, Doshisha University
  • NAKAJIMA Riichiro
    Department of Chemical Engineering and Materials Science, Faculty of Engineering, Doshisha University

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Abstract

A new immunoassay using chemiluminescence detection of dyestuff-containing liposomes as a labeling reagent is proposed. Human serum albumin labeled with Eosin Y containing-liposomes was employed as a labeling reagent for an immunoassay. After the immune reaction was carried out in the presence of an antibody-immobilized glass bead, the reactant solution was subjected to the capillary electrophoresis-hemiluminescence detection system. That is, the bound/free separation was conducted by use of glass beads, also, other coexisting compounds which might influence chemiluminescence detection were easily separated from the labeled human serum albumin by capillary electrophoresis. The labeled human serum albumin in the reactant solution was detected with high sensitivity. The amount of the labeled human serum albumin showed a good relationship to that of human serum albumin as an analyte through immune reaction. Human serum albumin could be determined over the range of 1×10-6 - 5×10-4 M. The present method was also applicable to the determination of protein in a serum sample without being interfered with by coexisting constituents.

Journal

  • Analytical Sciences

    Analytical Sciences 16 (2), 121-124, 2000

    The Japan Society for Analytical Chemistry

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