Single-Cell Imaging of the Ca〔2+〕 Influx into Bovine Endothelial Cells Occurring in Response to an Alternating Electric Stimulus

MATSUOKA Hideaki, HASHIMOTO Kenji, HORIKIRI Shigetoshi, SAITO Mikako, YAMAMOTO Kimiko, ANDO Joji

doi:10.2116/analsci.18.1205

Single-Cell Imaging of the Ca〔2+〕 Influx into Bovine Endothelial Cells Occurring in Response to an Alternating Electric Stimulus

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MATSUOKA Hideaki

Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology
HASHIMOTO Kenji

Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology
HORIKIRI Shigetoshi

Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology
SAITO Mikako

Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology
YAMAMOTO Kimiko

Department of Biomedical Engineering, Graduate School of Medicine, University of Tokyo
ANDO Joji

Department of Biomedical Engineering, Graduate School of Medicine, University of Tokyo

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タイトル別名

Single-Cell Imaging of the Ca2+ Influx into Bovine Endothelial Cells Occurring in Response to an Alternating Electric Stimulus.

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説明

The electric control of cellular functions via Ca²⁺ was formerly suggested. From this viewpoint, the involvement of a Ca²⁺ channel was studied using bovine fetal arterial endothelial (BFAE) cells in which P2X4, an ATP-operated and fluid shear stress sensitive Ca²⁺ channel, exists predominantly. An electric stimulus (sine wave, 10 Hz, 10 V_PP, 30 s) caused a marked influx of Ca²⁺ into BFAE cells from an extracellular solution. The magnitude of the [Ca²⁺]_i change increased with a decrease in the frequency in the range from 100 Hz to 5 Hz. Regarding the pathway of this Ca²⁺ influx, single-cell imaging and an ATP depletion experiment strongly suggested the involvement of a pathway different from P2X4. This pathway was thought to be a non-specific one, because typical Ca²⁺ channel blockers, such as verapamil, Gd³⁺, and Co²⁺, could not inhibit the Ca²⁺ influx.