Voltammetric Evaluation of the Binding between Wheat Germ Agglutinin and Thionine/Glucose-modified Magnetic Microbeads
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- SUGAWARA Kazuharu
- Faculty of Education, Gunma University
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- SENBONGI Atsushi
- Faculty of Education, Gunma University
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- KAMIYA Naoto
- Faculty of Education, Gunma University
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- HIRABAYASHI George
- Faculty of Education, Gunma University
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- KURAMITZ Hideki
- Department of Environmental Biology and Chemistry, Faculty of Science, University of Toyama
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Abstract
The binding between glucose residues and wheat germ agglutinin (WGA) on thionine/glucose-modified magnetic microbeads was evaluated using voltammetry. Thionine is an electroactive compound and has two amino groups. Thionine was immobilized to magnetic beads via cross-linking of the amino groups on the beads with an amino group on thionine. Glucose was bound to the other amino group of thionine via the formation of a Schiff base. The beads were only several micrometers in size the same size, as cells. WGA-binding to glucose on the bead surface blankets the thionine moiety. Thus, WGA-binding could be detected as a decrease in peak current of the thionine moiety.
Journal
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- Analytical Sciences
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Analytical Sciences 24 (6), 717-720, 2008
The Japan Society for Analytical Chemistry
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Details 詳細情報について
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- CRID
- 1390001204256016768
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- NII Article ID
- 10025204221
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- NII Book ID
- AA10500785
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- ISSN
- 13482246
- 09106340
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- NDL BIB ID
- 9521997
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed