Analyses of Anandamide and Endocannabinoid-like Compounds Using Collision-induced Dissociation in Fast Atom Bombardment Ionization-Mass Spectrometry and Gas Chromatography/Chemical Ionization-Mass Spectrometry
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- KASAI Hiroko F.
- Faculty of Pharmaceutical Sciences, Hoshi University
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- TSUBUKI Masayoshi
- Faculty of Pharmaceutical Sciences, Hoshi University
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- TAKAHASHI Kazunori
- Faculty of Pharmaceutical Sciences, Hoshi University
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- HONDA Toshio
- Faculty of Pharmaceutical Sciences, Hoshi University
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- UEDA Haruhisa
- Faculty of Pharmaceutical Sciences, Hoshi University
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Abstract
The utility of the collision-induced dissociation (CID) of two different forms of precursor cations generated by the fast atom bombardment (FAB) ionization of N-arachidonylethanolamine (anandamide) and a series of endocannabinoid-like compounds, such as N-oleoylethanolamine, N-palmitoylethanolamine, N-stearoylethanolamine, N-linoleoylethanolamine, N-oleoylpropanolamine, and N-palmitoylpropanolamine, as a method of providing general information on their characterizations was examined. The CID spectra of lithium-adduct [M+Li]+ ions of the amines with unsaturated hydrocarbon chains were rich in structurally informative charge-site-remote (CSR) fragmentation patterns that provide information on the locations of double bonds in hydrocarbon chains. On the other hand, the CID reactions of [M+H]+ ions produced acylium ions that are derived from the cleavage of amide bonds, thus providing information on the size of the hydrocarbon chains, although CSR fragmentations were not observed. These compounds without derivatization were analyzed using gas chromatography/chemical ionization-mass spectrometry (GC/CI-MS) with a polyethylene glycol phased column with fused silica capillary pre-tubing. Identifiable molecular-related [M+H]+ ions were observed.
Journal
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- Analytical Sciences
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Analytical Sciences 19 (12), 1593-1598, 2003
The Japan Society for Analytical Chemistry
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Details 詳細情報について
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- CRID
- 1390001204256218496
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- NII Article ID
- 130004440675
- 10011873999
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- NII Book ID
- AA10500785
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- COI
- 1:CAS:528:DC%2BD3sXpvV2ntr4%3D
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- ISSN
- 13482246
- 09106340
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- NDL BIB ID
- 6774666
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- PubMed
- 14696920
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed