Enhancement of the Binding Ability of a Ligand for Nucleobase Recognition by Introducing a Methyl Group

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  • DAI Qing
    Department of Chemistry, Graduate School of Science, Tohoku University, and CREST, Japan Science and Technology Agency (JST)
  • XU Chun-Yan
    Department of Chemistry, Graduate School of Science, Tohoku University, and CREST, Japan Science and Technology Agency (JST)
  • SATO Yusuke
    Department of Chemistry, Graduate School of Science, Tohoku University, and CREST, Japan Science and Technology Agency (JST)
  • YOSHIMOTO Keitaro
    Bioengineering Laboratory, RIKEN (The Institute of Physical and Chemical Research)
  • NISHIZAWA Seiichi
    Department of Chemistry, Graduate School of Science, Tohoku University, and CREST, Japan Science and Technology Agency (JST)
  • TERAMAE Norio
    Department of Chemistry, Graduate School of Science, Tohoku University, and CREST, Japan Science and Technology Agency (JST)

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Abstract

The recognition ability of pteridine derivatives for nucleobases opposite an abasic (AP) site in an oligodeoxynucleotide (ODN) duplex is enhanced by using a propylene residue (Spacer-C3) as an AP site. The recognition ability is further enhanced both by attaching methyl groups to a fluorescent ligand and by measuring the fluorescence response at 5°C; 6.2 × 106 M-1 of the binding constant is attained between 2-amino-6,7-dimethyl-4-hydroxypteridine and guanine opposite the AP site in water.

Journal

  • Analytical Sciences

    Analytical Sciences 22 (2), 201-203, 2006

    The Japan Society for Analytical Chemistry

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