Characterization of Metal Binding Properties of Rhamnogalacturonan II from Plant Cell Walls by Size-Exclusion HPLC/ICP-MS
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- MATSUNAGA Toshiro
- National Agricultural Research Center for Kyushu Okinawa Region
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- ISHII Tadashi
- Forestry and Forest Products Research Institute
Bibliographic Information
- Other Title
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- Characterization of Metal Binding Properties of Rhamnogalacturonan 2 from Plant Cell Walls by Size-Exclusion HPLC/ICP-MS
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Abstract
The binding properties of metal ions to a pectic polysaccharide, rhamnogalacturonan II (RG-II), from plant cell walls were analyzed by size-exclusion HPLC/ICP-MS. The dimeric RG-II borate complex (dRG-II-B) from sugar beet and red wine contained 0.8 - 1.1 mol/mol of B, 0.8 - 1.1 mol/mol of Ca, 0.1 - 0.3 mol/mol of Sr, and 0.03 - 0.07 mol/mol of Ba. The treatment of dRG-II-B with Sr2+, Ba2+, Pb2+ or La3+ exchanged the originally present Ca, Sr and Ba. In contrast, monomeric RG-II (mRG-II), which contained ∼0.1 mol/mol of Ca, formed complexes with La3+, Eu3+, and Lu3+ added to the solution, but did not do so with Ca2+, Sr2+, Ba2+ and Pb2+. The HPLC/ICP-MS and HPLC/RI (refractive index detector) analysis of the partially hydrolyzed mRG-II that was treated with La3+ indicated that the side chains and backbone of mRG-II together form a lanthanoid binding site.
Journal
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- Analytical Sciences
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Analytical Sciences 20 (10), 1389-1393, 2004
The Japan Society for Analytical Chemistry
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Keywords
Details 詳細情報について
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- CRID
- 1390001204256349824
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- NII Article ID
- 10013657249
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- NII Book ID
- AA10500785
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- COI
- 1:CAS:528:DC%2BD2cXovVeksb4%3D
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- ISSN
- 13482246
- 09106340
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- NDL BIB ID
- 7111337
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- PubMed
- 15524188
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed