Electrochemical Immunosensor for Carcinoembryonic Antigen Based on Antigen Immobilization in Gold Nanoparticles Modified Chitosan Membrane

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  • LIN Jiehua
    College of Chemistry and Molecular Engineering, Qingdao University of Science & Technology
  • QU Wei
    College of Chemistry and Molecular Engineering, Qingdao University of Science & Technology
  • ZHANG Shusheng
    College of Chemistry and Molecular Engineering, Qingdao University of Science & Technology

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抄録

A disposable electrochemical immunosensor for carcinoembryonic antigen (CEA) was proposed based on the antigen immobilized in a colloidal gold nanoparticles modified chitosan membrane on the surface of an indium-tin oxide (ITO) electrode. The different membranes were characterized by scanning electron microscope and electrochemical methods. Based on a competitive immunoassay format, the immobilized antigen of the immunosensor was incubated with a horseradish peroxidase (HRP) labeled antibody and sample CEA antigen, and the formed immunoconjugate in the immunosensor was detected by an o-phenylenediamine-H2O2-HRP electrochemical system. Under the optimal experimental conditions, the electrocatalytic current decreased linearly with the competitive mechanism. CEA could be determined in the linear range from 2.0 to 20 ng/ml with a detection limit of 1.0 ng/ml. The prepared CEA immunosensor is not only economic due to the low-cost ITO electrode obtained from industrial mass production, but is also capable with good stability and reproducibility for batch fabrication.

収録刊行物

  • Analytical Sciences

    Analytical Sciences 23 (9), 1059-1063, 2007

    社団法人 日本分析化学会

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