DNA-Conjugated Polymers for Self-Assembled DNA Chip Fabrication
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- TAIRA Shu
- Research Center of Advanced Bionics, National Institute of Advanced Industrial Science and Technology School of Materials Science, Japan Advanced Institute of Science and Technology
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- YOKOYAMA Kenji
- Research Center of Advanced Bionics, National Institute of Advanced Industrial Science and Technology
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Abstract
We developed two DNA-conjugated polymers, one based on polyallylamine and the other on polyacrylic acid, for use in DNA chips. A 30-mer single-stranded DNA probe and thioctic acid were covalently attached to polyallylamine as sidechains. The same single-stranded DNA and 3-(pyridyldithio)propionyl hydrazide were covalently attached to polyacrylic acid as sidechains. Both DNA-conjugated polymers could be specifically immobilized onto a gold sensor substrate by a self-assembly technique. The interactions between fully matched DNA and each DNA-conjugated polymer were investigated by surface plasmon resonance. A gold surface modified with either DNA-conjugated polymer recognized fully matched DNA much better than unmatched DNA. The hybridization selectivity and efficiency of DNA-conjugated polyallylamine was optimized by adjusting the pH so as to reduce the effects of cationic polymer sidechains. The hybridization selectivity and efficiency of DNA-conjugated polymers were higher than those of a conventional immobilized thiol-based DNA. The coating of DNA-conjugated polymers reduced nonspecific adsorption of DNA by the gold substrate. DNA-conjugated polyacrylic acid was more selective toward fully matched DNA than was DNA-conjugated polyallylamine. Therefore, DNA-conjugated polymers show promise for application in novel DNA chips.
Journal
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- Analytical Sciences
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Analytical Sciences 20 (2), 267-271, 2004
The Japan Society for Analytical Chemistry
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Details 詳細情報について
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- CRID
- 1390001204256409472
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- NII Article ID
- 10012046671
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- NII Book ID
- AA10500785
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- COI
- 1:CAS:528:DC%2BD2cXivVClsLY%3D
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- ISSN
- 13482246
- 09106340
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- NDL BIB ID
- 6843062
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- PubMed
- 15055949
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed