Voltammetric behaviors of wheat-germ agglutinin on a chitin-modified carbon-past electrode
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- SUGAWARA Kazuharu
- Faculty of Education, Gunma University
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- TERAUCHI Aiko
- Faculty of Education, Gunma University
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- KAMIYA Naoto
- Faculty of Education, Gunma University
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- HIRABAYASHI George
- Faculty of Education, Gunma University
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- KURAMITZ Hideki
- Department of Environmental Biology and Chemistry, Faculty of Science, University of Toyama
書誌事項
- タイトル別名
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- Voltammetric Behaviors of Wheat-Germ Agglutinin on a Chitin-modified Carbon-Paste Electrode
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説明
The voltammetric behavior of wheat-germ agglutinin (WGA) on a chitin-modified carbon-paste electrode (CPE) was investigated using glucose labeled with an electroactive compound. WGA usually consists of two subunits, each with two binding sites for sugars. WGA was immobilized on the electrode surface by selective binding to a N-acetylglucosamine residue of chitin. Because glucose also combines with WGA, the glucose was coupled with electroactive daunomycin to evaluate the binding. When a WGA-labeled glucose complex was formed, the electroactive moiety became electroinactive. The binding caused a decrease in the peak current of the labeled glucose. In a measurement of only daunomycin used as a label, the peak current in a solution with WGA was similar to that in a solution without WGA. Therefore, it is clear that the labeled glucose was held in the remaining binding site of WGA on the electrode surface. Thus, a CPE modified with chitin would be powerful as a reaction field between sugar and lectin.
収録刊行物
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- Analytical Sciences
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Analytical Sciences 24 (5), 583-587, 2008
社団法人 日本分析化学会
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詳細情報 詳細情報について
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- CRID
- 1390001204256667264
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- NII論文ID
- 130004441647
- 10021099963
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- NII書誌ID
- AA10500785
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- ISSN
- 13482246
- 09106340
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- NDL書誌ID
- 9483014
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- PubMed
- 18469462
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- 本文言語コード
- en
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- NDLサーチ
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