Interaction of Bis(ethylene)tin(bis(salicylidene)ethylenediamine) with DNA.

DOI PDF Web Site Web Site Web Site View 1 Remaining Hide 38 References
  • LIU Guo-Dong
    Department of Chemistry, East China Normal University
  • LIAO Jian-Pei
    College of Chemistry and Chemical Engineering, Hunan University
  • FANG Yu-Zhi
    Department of Chemistry, East China Normal University
  • HUANG Sha-Sheng
    College of Chemistry and Chemical Engineering, Hunan University
  • SHENG Guo-Li
    College of Chemistry and Chemical Engineering, Hunan University
  • YU Ru-Qin
    College of Chemistry and Chemical Engineering, Hunan University

Search this article

Abstract

The fluorescence spectral characteristics and interaction of bis(ethylene)tin(bis(salicylidene)ethylenediamine)[Et2Sn(salen)] with DNA are described. The polarity of the solvent has a strong effect on the fluorescence characteristics of Et2Sn(salen). Et2Sn(salen) bound to DNA showed a marked decrease in the fluorescence intensity with a bathochromic shift of the excitation and emission peaks. A hypochromism in the UV absorption spectra was also observed. KI quenching and competitive binding to DNA between Et2Sn(salen) and ethidium bromide (EB) were studied in connection with other experimental observations to show that the interactive model between Et2Sn(salen) and DNA is an intercalative one. The pH and salt effect on the fluorescence properties was also investigated. The intrinsic binding constant was estimated to be 1.071 × 105 mol L-1 in base pairs and the binding site number is 1.98, respectively. A linear relationship between F/F0 and the concentration of calf thymus DNA covers 5.1 × 10-6 - 2.41 × 10-4 mol L-1, which can be utilized for determining traces of calf thymus DNA with a detection limit of 1.1 × 10-7 mol L-1 in base pairs.

Journal

  • Analytical Sciences

    Analytical Sciences 18 (4), 391-395, 2002

    The Japan Society for Analytical Chemistry

References(38)*help

See more

Keywords

Details 詳細情報について

Report a problem

Back to top