Quantitative Measurement of 17.BETA.-Estradiol and Estriol in River Water by Time-Resolved Fluoroimmunoassay.

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  • MAJIMA Keisuke
    Department of Chemistry, Waseda University, Advanced Research Institute for Science and Engineering
  • FUKUI Takashi
    Department of Chemistry, Waseda University, Advanced Research Institute for Science and Engineering
  • YUAN Jingli
    Department of Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences
  • WANG Guilan
    Department of Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences
  • MATSUMOTO Kazuko
    Department of Chemistry, Waseda University, Advanced Research Institute for Science and Engineering

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Other Title
  • Quantitative Measurement of 17β-Estradiol and Estriol in River Water by Time-Resolved Fluoroimmunoassay
  • Quantitative Measurement of 17 ベータ Estradiol and Estriol in River Water by Time Resolved Fluoroimmunoassay

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Abstract

A sensitive method for detecting 17β-estradiol (E2) and estriol (E3) in river water has been developed, based on the time-resolved fluoroimmunoassay by using a fluorescent europium chelate label, 4,4′-bis(1″,1″,1″,2″,2″,3″,3″-heptafluoro-4″,6″-hexanedion-6″-yl)-chlorosulfo-o-terphenyl (BHHCT)-Eu3+. In the E2 assay, microtiter plates were coated with the E2-bovine serum albumin (BSA) conjugate. The anti-17β-estradiol antibody, the biotinylated goat anti-rabbit IgG antibody and the BHHCT-Eu3+ labeled streptavidin (SA)-BSA conjugate were used. In the E3 assay, the goat anti-rabbit IgG antibody was coated on a microtiter plate. The anti-estriol antibody and the BHHCT-Eu3+ labeled E3-BSA conjugate were used. The detection limits for E2 and E3 were 2.3 pg/ml and 4.3 pg/ml, respectively, and the analytical recoveries were 95 - 120%. Quantitative measurement of estrogens in river water was carried out for Kanda River (Tokyo, Japan) by using the method. The E2 and E3 levels were 32 pg/ml and 5.5 pg/ml, respectively. The detection limits of the present method are in the same orders of magnitude as those of ELISA for E2, and are 1 - 2 orders of magnitude better for E3.

Journal

  • Analytical Sciences

    Analytical Sciences 18 (8), 869-874, 2002

    The Japan Society for Analytical Chemistry

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