Reversed-Phase HPLC of Monosaccharides in Glycoproteins Derivatized with Aminopyrazine with Fluorescence Detection.

DOI PDF Web Site Web Site Web Site 2 Citations 52 References
  • WU Wei
    Graduate School of Pharmaceutical Sciences, Kyushu University
  • HAMASE Kenji
    Graduate School of Pharmaceutical Sciences, Kyushu University
  • KIGUCHI Masaru
    Graduate School of Pharmaceutical Sciences, Kyushu University
  • YAMAMOTO Koji
    Graduate School of Pharmaceutical Sciences, Kyushu University
  • ZAITSU Kiyoshi
    Graduate School of Pharmaceutical Sciences, Kyushu University

Search this article

Abstract

A precise method for the determination of monosaccharides is described. Monosaccharides were separated as their aminopyrazine derivatives using reversed-phase HPLC and were detected at 410 nm by a fluorescence detector with excitation at 245 nm. The 8 monosaccharides tested were completely separated in about 20 min, and the detection limits were 45 - 800 fmol. Particularly, strong fluorescence was observed for N-acetylated monosaccharide and sensitive determination could be carried out. The monosaccharide composition of several glycoproteins was also determined by reversed-phase HPLC and anion-exchange HPLC, and the results were consistent with the reported values.

Journal

  • Analytical Sciences

    Analytical Sciences 16 (9), 919-922, 2000

    The Japan Society for Analytical Chemistry

Citations (2)*help

See more

References(52)*help

See more

Keywords

Details 詳細情報について

Report a problem

Back to top