Determination of Nucleic Acids Using Calcein-Neodymium Complex as a Fluorescence Probe.
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- ZHU Zhancai
- Department of Chemistry, Zhangzhou Teacher’s University
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A novel fluorometric method has been developed for rapid determination of DNA and RNA with calcein-neodymium complex as a fluorescence probe. The method is based on the fluorescence enhancement of calcein-Nd(III) complex in the presence of DNA or RNA, with maximum excitation and emission wavelength at 489 nm and 514 nm, respectively. Under optimal conditions, the calibration graphs are linear over the range 0.5 - 3.0 μg/ml for both DNA and yeast RNA, 0.4 - 2.0 μg/ml for fish sperm DNA (FS DNA) and 0 - 3.0 μg/ml for calf thymus DNA (CT DNA). The corresponding detection limits are 15.1 ng/ml for DNA, 21.2 ng/ml for yeast RNA, 10.5 ng/ml for FS DNA and 8.9 ng/ml for CT DNA. The interaction mechanism for the binding of calcein-Nd(III) complex to DNA is also studied. The results of absorption spectra, fluorescence polarization measurements and thermal denaturation experiments, suggested that the interaction between calcein-Nd(III) complex and DNA is an electrostatic interaction.
収録刊行物
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- Analytical Sciences
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Analytical Sciences 17 (12), 1375-1377, 2001
社団法人 日本分析化学会
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詳細情報 詳細情報について
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- CRID
- 1390001204257050112
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- NII論文ID
- 130004440357
- 10007419745
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- NII書誌ID
- AA10500785
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- COI
- 1:CAS:528:DC%2BD38XksF2r
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- ISSN
- 13482246
- 09106340
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- NDL書誌ID
- 6018688
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- PubMed
- 11783784
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 使用不可