Determination of Total Mercury and Methylmercury in Human Hair by Graphite-Furnace Atomic Absorption Spectrophotometry Using 2,3-Dimercaptopropane-1-sulfonate as a Complexing Agent.
-
- CHEN Wen-Yi
- Department of Environmental Science, Tunghai University
-
- WANG Yu-Chun
- Department of Environmental Science, Tunghai University
-
- KUO Mao-Sung
- Department of Environmental Science, Tunghai University
この論文をさがす
抄録
For the determination of total mercury in hair, an amount (25.0 mg) of hair sample was digested with conc. HNO3 (400 µl) at 90°C for 10 min in a 7-ml teflon microreaction vessel. After digestion, the pH of the acidic hair mixture was adjusted to 5.0 - 6.0 by NaOH and was then passed through a clean-up Sep-Pak C18 cartridge. To the eluate, 2,3-dimercaptopropane-1-sulfonate (DMPS) and sodium acetate buffer (pH = 6.0) were added to form a mercury-DMPS complex. This complex was preconcentrated on two Sep-Pak C18 cartridges in series, and each cartridge was eluted with methanol and adjusted to 2.00 ml. A portion (50 µl) was introduced into a graphite cuvette and then atomized according to a temperature program. The method detection limit (MDL, 3 σ) was 0.064 (µg g-1); the calibration graph was linear up to 7.52 µg g-1. Good accuracies were obtained when testing two human hair certified reference materials (GBW 09101 and BCR-397). Six real samples were analyzed, and the recoveries were 95.8 - 98.2% with a relative standard deviation (RSD, n = 3) < 2.1%. For the determination of methylmercury (CH3Hg+), 25.0 mg of hair sample was extracted with 2.0 mol dm-3 HCl (1.0 ml) by ultrasonicating for 1 h. The supernatant solution was used for CH3Hg+ analysis and the hair residue was used for the analysis of inorganic mercury (Hg2+). The MDL of CH3Hg+ was 0.068 µg g-1; the calibration graph was linear up to 6.00 µg g-1. Six real samples were analyzed, and the recoveries were 96.0 - 99.2% with RSD (n = 3) < 2.3%. The sum of the concentrations of CH3Hg+ and Hg2+ was very close to that of the total mercury measured with a relative error within 3.6%. The proposed method can be accurately applied to the measurement of CH3Hg+, Hg2+, and total mercury in hair samples.
収録刊行物
-
- Analytical Sciences
-
Analytical Sciences 18 (3), 255-260, 2002
社団法人 日本分析化学会
- Tweet
キーワード
詳細情報 詳細情報について
-
- CRID
- 1390001204257079040
-
- NII論文ID
- 130004440540
- 10011313985
-
- NII書誌ID
- AA10500785
-
- COI
- 1:CAS:528:DC%2BD38XitFCmtro%3D
-
- ISSN
- 13482246
- 09106340
-
- NDL書誌ID
- 6262483
-
- PubMed
- 11918181
-
- Web Site
- http://id.ndl.go.jp/bib/6262483
- https://ndlsearch.ndl.go.jp/books/R000000004-I6262483
- https://link.springer.com/content/pdf/10.2116/analsci.18.255.pdf
- https://link.springer.com/article/10.2116/analsci.18.255/fulltext.html
- http://www.jstage.jst.go.jp/article/analsci/18/3/18_3_255/_pdf
- https://search.jamas.or.jp/link/ui/2002201303
-
- 本文言語コード
- en
-
- データソース種別
-
- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
-
- 抄録ライセンスフラグ
- 使用不可