Methods of Analysis for Chemicals that Promote/Disrupt Cellular Signaling.
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- UMEZAWA Yoshio
- Department of Chemistry, School of Science, The University of Tokyo
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- OZAWA Takeaki
- Department of Chemistry, School of Science, The University of Tokyo
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- SATO Moritoshi
- Department of Chemistry, School of Science, The University of Tokyo
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Abstract
Methods of analysis were presented for chemicals that promote or disrupt cellular signaling pathways. The developed analytical methods are based not only on receptor binding, but also on the following known molecular-level processes involved in signal transduction along signaling pathways, reconstituted in vitro or taken in part in living cells. The methods were discussed in relation to receptor binding assay and/or bioassay. Examples include: (1) Insulin signaling pathways; (1-i) Chemical selectivity of agonists for insulin signaling pathways based on agonist-induced phosphorylation of a target peptide; (1-ii) An SPR-based screening method for agonist selectivity for insulin signaling pathways based on the binding of phosphotyrosine to its specific binding protein; (1-iii) A fluorescent indicator for tyrosine phosphorylation-based insulin signaling pathways; (2) An optical method for evaluating ion selectivity for calcium signaling pathways in the cell; (3) Assay and screening of chemicals that disrupt cellular signaling pathways, potential endocrine disruptors in particular; (4) Protein conformational changes, and (5) A screening method for antigen-specific IgE using mast cells, based on intracellular calcium signaling.
Journal
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- Analytical Sciences
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Analytical Sciences 18 (5), 503-516, 2002
The Japan Society for Analytical Chemistry
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Details 詳細情報について
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- CRID
- 1390001204257243264
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- NII Article ID
- 130004440591
- 10011312842
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- NII Book ID
- AA10500785
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- COI
- 1:CAS:528:DC%2BD38XjvFGlu7o%3D
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- ISSN
- 13482246
- 09106340
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- NDL BIB ID
- 6263224
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- PubMed
- 12036116
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed