An Anti E. Coli O157:H7 Antibody-Immobilized Microcantilever for the Detection of Escherichia Coli (E. coli)
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- ZHANG Jing
- Department of Chemistry and the Institute for Micromanufacturing, Louisiana Tech University
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- JI Hai-Feng
- Department of Chemistry and the Institute for Micromanufacturing, Louisiana Tech University
Bibliographic Information
- Other Title
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- Anti E Coli O157 H7 Antibody Immobilized Microcantilever for the Detection of Escherichia Coli E coli
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Abstract
A silicon microcantilever sensor was developed for the detection of Escherichia coli O157:H7. The microcantilever was modified by anti-E. coli O157:H7 antibodies on the silicon surface of the cantilever. When the aquaria E. coli O157:H7 positive sample is injected into the fluid cell where the microcantilever is held, the microcantilever bends upon the recognition of the E. coli O157:H7 antigen by the antibodies on the surface of the microcantilever. A negative control sample that does not contain E. coli O157:H7 antigen did not cause any bending of the microcantilever. The detection limit of the sensor was 1 × 106 cfu/mL when the assay time was < 2 h.
Journal
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- Analytical Sciences
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Analytical Sciences 20 (4), 585-587, 2004
The Japan Society for Analytical Chemistry
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Details 詳細情報について
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- CRID
- 1390001204257308416
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- NII Article ID
- 10012850319
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- NII Book ID
- AA10500785
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- COI
- 1:CAS:528:DC%2BD2cXjsVGltL4%3D
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- ISSN
- 13482246
- 09106340
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- NDL BIB ID
- 6911913
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- PubMed
- 15116951
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed