An Anti E. Coli O157:H7 Antibody-Immobilized Microcantilever for the Detection of Escherichia Coli (E. coli)

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  • ZHANG Jing
    Department of Chemistry and the Institute for Micromanufacturing, Louisiana Tech University
  • JI Hai-Feng
    Department of Chemistry and the Institute for Micromanufacturing, Louisiana Tech University

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Other Title
  • Anti E Coli O157 H7 Antibody Immobilized Microcantilever for the Detection of Escherichia Coli E coli

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Abstract

A silicon microcantilever sensor was developed for the detection of Escherichia coli O157:H7. The microcantilever was modified by anti-E. coli O157:H7 antibodies on the silicon surface of the cantilever. When the aquaria E. coli O157:H7 positive sample is injected into the fluid cell where the microcantilever is held, the microcantilever bends upon the recognition of the E. coli O157:H7 antigen by the antibodies on the surface of the microcantilever. A negative control sample that does not contain E. coli O157:H7 antigen did not cause any bending of the microcantilever. The detection limit of the sensor was 1 × 106 cfu/mL when the assay time was < 2 h.

Journal

  • Analytical Sciences

    Analytical Sciences 20 (4), 585-587, 2004

    The Japan Society for Analytical Chemistry

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