Fluorescence Spectral Study of Interaction of Water-soluble Metal Complexes of Schiff-base and DNA.
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- LIU Guo-Dong
- College of Chemistry and Chemical Engineering, State Key Laboratory for Chemo/Biosensing and Chemometrics, Hunan University Department of Chemistry, East China Normal University
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- LIAO Jian-Pei
- College of Chemistry and Chemical Engineering, State Key Laboratory for Chemo/Biosensing and Chemometrics, Hunan University
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- HUANG Sha-Sheng
- College of Chemistry and Chemical Engineering, State Key Laboratory for Chemo/Biosensing and Chemometrics, Hunan University
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- SHEN Guo-Li
- College of Chemistry and Chemical Engineering, State Key Laboratory for Chemo/Biosensing and Chemometrics, Hunan University
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- YU Ru-Qin
- College of Chemistry and Chemical Engineering, State Key Laboratory for Chemo/Biosensing and Chemometrics, Hunan University
Bibliographic Information
- Other Title
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- Fluorescence Spectral Studuy of Interaction of Water-soluble Metal Complexes of Schiff-base and DNA
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Abstract
The fluorescence spectral characteristics and the interaction of several water-soluble metal complexes of Schiff-base with DNA are described. Among the complexes tested, Mn-Schiff-base bound to DNA showed a marked decrease in the fluorescence intensity with a blue shift of the excitation and emission peaks. Some hypochromism in the UV absorption spectra was also observed. KI quenching and competitive binding to DNA between Mn-Schiff-base and ethidium bromide (EB) were studied in connection with other experimental observations to show that the interactive model between Mn-Schiff-base and DNA is an intercalative one. The pH and salt effect on the fluorescence properties was also investigated. The linear relationship between F/F0 and the concentration of calf thymus DNA covers 3.0 × 10-6 - 2 × 10-4 mol L-1, which can be utilized for determining traces of calf thymus DNA with a detection limit of 8.0 × 10-7 mol L-1 in base pairs.
Journal
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- Analytical Sciences
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Analytical Sciences 17 (9), 1031-1036, 2001
The Japan Society for Analytical Chemistry
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Details 詳細情報について
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- CRID
- 1390001204257371008
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- NII Article ID
- 10007177577
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- NII Book ID
- AA10500785
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- COI
- 1:CAS:528:DC%2BD3MXntFWqtbo%3D
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- ISSN
- 13482246
- 09106340
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- NDL BIB ID
- 5911550
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- PubMed
- 11708054
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed