Discrimination of the length of double-stranded DNA fragments by the bis-intercalating ligand.
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- TAKENAKA Shigeori
- Department of Chemical Systems and Engineering, Graduate School of Engineering, Kyushu University
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- FUNATU Yosuke
- Department of Biochemical Engineering and Science, Kyushu Institute of Technology
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- SHIGEMOTO Naonori
- Department of Biochemical Engineering and Science, Kyushu Institute of Technology
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- KONDO Hiroki
- Department of Biochemical Engineering and Science, Kyushu Institute of Technology
Bibliographic Information
- Other Title
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- Discrimination of the Length of Double-
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Description
Bis-intercalator 2 with two acridine moieties separated by a bis(hexamethylene)viologen linker was synthesized and its binding of calf thymus DNA was studied. Bis-intercalator 2 was designed to cover about a 24 Å stretch of double stranded DNA. The binding constant of bis-intercalator 2 for calf thymus DNA was 1.7x105M-1 in 10mM MES, 1mM EDTA and 0.1M NaCl (pH 6.25). The two acridine units of 2 stacked with each other in solution and upon its binding the structure of DNA was perturbed considerably. The binding affinities of the bis-intercalator for DNA duplex fragments of different length, d(AT)n where n=3-8, were estimated by its effect on the DNA melting temperature. It was found that d(AT)5 with a length of 34 Å was stabilized most by 2. By contrast, quinacrine 1 did not show any stabilizing effect on these DNAs. These results suggest that the bis-intercalator was capable of discriminating the length of double-stranded DNA fragments precisely. This ability of 2 may be applied for the precise separation of double-stranded DNA fragments according to their length.
Journal
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- Analytical Sciences
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Analytical Sciences 13 (Supplement), 177-180, 1997
The Japan Society for Analytical Chemistry
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Details 詳細情報について
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- CRID
- 1390001204257506560
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- NII Article ID
- 130003528382
- 40005334976
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- NII Book ID
- AA10500785
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- ISSN
- 13482246
- 09106340
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- NDL BIB ID
- 4371156
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed