Highly Sensitive Detection of Bensulfuron-methyl by Time-Resolved Fluoroimmunoassay Using a Tetradentate .BETA.-Diketonate Europium Chelate as a Label.
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- YUAN Jingli
- Department of Chemistry, Waseda University Japan Science and Technology Corporation (JST)
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- WANG Guilan
- Department of Chemistry, Waseda University Japan Science and Technology Corporation (JST)
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- KIMURA Hiroko
- Japan Science and Technology Corporation (JST) Department of Forensic Medicine, Juntendo University School of Medicine
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- MATSUMOTO Kazuko
- Department of Chemistry, Waseda University Japan Science and Technology Corporation (JST)
Bibliographic Information
- Other Title
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- Highly Sensitive Detection of Bensulfuron-methyl by Time-Resolved Fluoroimmunoassay Using a Tetradentate β-Diketonate Europium Chelate as a Label
- Highly Sensitive Detection of Bensulfuron-methyl by Time-Resolved Fluoroimmunoassay Using a Tetradentate ベーターDiketonate Europium Chelate as a Label
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Abstract
A tetradentate β-diketonate europium fluorescent chelate, 4,4′-bis(1″,1″,1″,2″,2″,3″,3″-heptafluoro-4″,6″-hexanedion-6″-yl)-chlorosulfo-o-terphenyl (BHHCT)-Eu3+, was used as a label for highly sensitive time-resolved fluoroimmunoassay of bensulfuron-methyl (BSM). A BSM-BSA (BSA=bovine serum albumin) conjugate was labeled with BHHCT-Eu3+ and the labeled BSM-BSA conjugate was used for competitive immunoassay. The assay was carried out in a 96-well microtiter plate coated with the anti-BSM monoclonal antibody. The method gives the detection limit of 24 pg/ml, which is one to two orders of magnitude lower than those of the conventional HPLC and other methods. The RSD of the present method is less than 10%, and the recovery is in the range of 90 - 110% for BSM water samples.
Journal
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- Analytical Sciences
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Analytical Sciences 15 (2), 125-128, 1999
The Japan Society for Analytical Chemistry
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Details 詳細情報について
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- CRID
- 1390001204257841152
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- NII Article ID
- 10002418608
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- NII Book ID
- AA10500785
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- COI
- 1:CAS:528:DyaK1MXhslansL8%3D
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- ISSN
- 13482246
- 09106340
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- NDL BIB ID
- 4648251
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed