Determination of Peroxidase Encapsulated in Liposomes Using Homogentisic Acid γ-Lactone Chemiluminescence

DOI HANDLE PDF Web Site Web Site ほか2件をすべて表示 一部だけ表示 参考文献30件 オープンアクセス

書誌事項

タイトル別名
  • Determination of Peroxidase Encapsulated in Liposomes Using Homogentisic Acid .GAMMA.-Lactone Chemiluminescence
  • Determination of Peroxidase Encapsulated in Liposomes Using Homogentisic Acid ガンマ Lactone Chemiluminescence

この論文をさがす

抄録

Homogentisic acid γ-lactone (HAL) chemiluminescence (CL) was applied to the determination of horseradish peroxidase (HRP) encapsulated in liposomes. HRP was detected after the lysis of HRP-trapped liposomes with Triton X-100. CL response rate, detection limit and linear range of calibration curve for HRP in HAL CL were compared with those in p-iodophenol (p-IP)-enhanced luminol CL. Maximal light emission in HAL CL appeared more rapidly compared to that in p-IP enhanced luminol CL, thus resulting in remarkable reduction of CL measurement time. The detection limit for HRP in HAL CL was the same as that in p-IP-enhanced luminol CL. The linear range of calibration curve for HRP in HAL CL was improved by a factor of 50 compared with that in p-IP-enhanced luminol CL. From these results, it was found that HAL CL were superior to p-IP-enhanced luminol CL for the determination of HRP encapsulated in liposomes.

収録刊行物

  • Analytical Sciences

    Analytical Sciences 21 (6), 701-704, 2005

    社団法人 日本分析化学会

参考文献 (30)*注記

もっと見る

関連プロジェクト

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ