Piezoelectric Quartz Crystal Impedance Study of the Pb2+-induced Precipitation of Bovine Serum Albumin and Its Dissolution with EDTA in an Aqueous Solution.

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  • YUAN Yu
    Chemical Research Institute, Hunan Normal University
  • CAI Yan
    Chemical Research Institute, Hunan Normal University
  • XIE Qingji
    Chemical Research Institute, Hunan Normal University
  • YAO Shouzhuo
    Chemical Research Institute, Hunan Normal University

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  • Piezoelectric Quartz Crystal Impedance Study of the Pb〔2+〕-induced Precipitation of Bovine Serum Albumin and Its Dissolution with EDTA in an Aqueous Solution

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Abstract

The piezoelectric quartz crystal impedance technique (QCI) was employed to monitor in situ the Pb2+-induced precipitation of BSA onto a gold electrode and the precipitate dissolution with EDTA in an aqueous solution. The critical precipitation concentration of Pb2+, at which the resonant frequency decreased significantly, was estimated to be 4.78 × 10-4 mol/L. The saturated adherence of the precipitate on the electrode was observed when the concentration of Pb2+ was greater than 7.53 × 10-2 mol/L. The frequency response was mainly caused by the mass effect of the precipitate adherence to the electrode, rather than the changes in the physico-chemical properties of the contacting liquid. An excess addition of Na2EDTA after the Pb2+-BSA dissolution led to new precipitation, probably due to the formation of an EDTA precipitate in this medium (pH≈3). The pH effect on the response of the resonant frequency was analyzed by using the sum of two exponential functions. A larger frequency response occurred at a pH greater than pI. These findings have been reasonably explained. Also, a decrease in the concentration of the background electrolyte increased the frequency response.

Journal

  • Analytical Sciences

    Analytical Sciences 18 (7), 767-771, 2002

    The Japan Society for Analytical Chemistry

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