Amperometric Glucose Biosensor Based on Mediated Electron Transfer between Immobilized Glucose Oxidase and Plasmapolymerized Thin Film of Dimethylaminomethylferrocene on Sputtered Gold Electrode

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  • KASE Yoshihiro
    Department of Electronic Engineering, Shibaura Institute of Technology
  • MUGURUMA Hitoshi
    Department of Electronic Engineering, Shibaura Institute of Technology

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Abstract

We propose an electron transfer-mediated amperometric enzyme biosensor based on plasma-polymerized thin film of dimethylaminomethylferrocene (DMAMF) on a sputtered gold electrode. The DMAMF plasma-polymerized film is deposited directly onto the surface of the electrode under dry conditions. The resulting thin film not only has redox sites but also is extremely thin (∼20 nm), adheres well onto the substrate (electrode), has a flat surface and a highly-crosslinked network structure, and is hydrophilic in nature. Glucose oxidase is densely immobilized onto the surface of DMAMF plasma-polymerized film on the gold electrode. From the electrochemical measurement, the biosensor can cover the wide range of glucose concentration (1.3 - 81 mM) at +350 mV of applied potential. The current response of the glucose biosensor was decreased by less than 5% in an aerobic solution as compared to that in an anaerobic solution. These show that the DMAMF plasma-polymerized films play a role as the electron transfer mediators between the reaction center of enzyme and the electrode.

Journal

  • Analytical Sciences

    Analytical Sciences 20 (8), 1143-1146, 2004

    The Japan Society for Analytical Chemistry

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