Single Molecule Detection by Laser Two-Photon Excited Fluorescence in a Capillary Flowing Cell.

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  • Single Molecule Detection by Laser Two-

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The two-photon excited fluorescence of fluorescein and Rhodamine B in a flowing sample cell was observed using a self mode-locked Ti-sapphire laser (180 fs pulse). The detection limit of fluorescein was less than 2 molecules in the probe volume. The single molecule detection of Rhodamine was successfully performed in a flowing cell, and the number of photon bursts agreed approximately with that expected.

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  • Analytical Sciences

    Analytical Sciences 14 (5), 913-916, 1998

    社団法人 日本分析化学会

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