MALDI Mass Spectrometry Using 2,4,6-Trihydroxyacetophenone and 2,4-Dihydroxyacetophenone with Cyclodextrins: Suppression of Matrix-related Ions in Low-molecular-weight Region
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- FUJITA Takashi
- Department of Chemistry, Graduate School of Science and Engineering, Tokyo Metropolitan University
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- FUJINO Tatsuya
- Department of Chemistry, Graduate School of Science and Engineering, Tokyo Metropolitan University
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- HIRABAYASHI Kazunori
- Department of Chemistry, Graduate School of Science and Engineering, Tokyo Metropolitan University
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- KORENAGA Takashi
- Department of Chemistry, Graduate School of Science and Engineering, Tokyo Metropolitan University
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Abstract
Matrix-assisted laser desorption/ionization (MALDI) mass spectrometry of a model peptide, Substance P (SubP), was carried out using 2,4,6-trihydroxyacetophenone (THAP) and 2,4-dihydroxyacetophenone (DHAP) with cyclodextrins (cyclodextrin-supported matrix). It was found that the use of a cyclodextrin-supported matrix simplified the mass spectrum in the low-molecular-weight region. The interaction between THAP/DHAP and cyclodextrin (CD) was studied by UV-vis absorption spectroscopy and the incorporation of matrix molecules into the cyclodextrin cavity was confirmed by 1H-NMR spectroscopy. DHAP showed tight incorporation with βCD (βCD(DHAP)) rather than THAP and it was found that the matrix-related peaks could be weakened by less than one third of the peak intensity of a protonated analyte. The βCD(DHAP) matrix was applied to the measurements of two low-molecular-weight compounds; adenosine and adrenaline. It became clear that the cyclic structure of the CD and the host-guest interaction between βCD and the matrix molecule were important to reduce the matrix-related peaks of THAP and DHAP.
Journal
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- Analytical Sciences
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Analytical Sciences 26 (7), 743-748, 2010
The Japan Society for Analytical Chemistry
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Keywords
Details 詳細情報について
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- CRID
- 1390001204258357504
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- NII Article ID
- 10026784351
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- NII Book ID
- AA10500785
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- COI
- 1:STN:280:DC%2BC3cnntlSntw%3D%3D
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- ISSN
- 13482246
- 09106340
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- NDL BIB ID
- 10744392
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- PubMed
- 20631433
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed