A New Analytical Procedure for Assay of Lysozyme in Human Tear and Saliva with Immobilized Reagents in Flow Injection Chemiluminescence System.
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- SONG Zheng-Hua
- Department of Chemistry, Northwest University
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- HOU Shuang
- Department of Chemistry, Northwest University
書誌事項
- タイトル別名
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- New Analytical Procedure for Assay of Lysozyme in Human Tear and Saliva with Immobilized Reagents in Flow Injection Chemiluminescence System
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抄録
A novel analytical procedure based on chemiluminescence (CL) detection was described for the determination of lysozyme at ng ml-1 level by using controlled-reagent-release technology in a flow injection system. The analytical reagents involved in the CL reaction, including luminol and periodate, were both immobilized on the anion-exchange resins in the flow injection system. Through water injection, luminol and periodate were eluted from the anion-exchange column to generate the chemiluminescence, which was inhibited in the presence of lysozyme. By measuring the decrease of CL intensity, one could analyze the lysozyme quantitatively. The decrement of CL emission was linear over the logarithm of lysozyme concentration in the range of 30 - 1000 ng ml-1. A typical analytical procedure, including sampling and washing, could be performed in 0.5 min at a flow rate of 2.0 ml min-1, giving a throughput of 120 h-1, with a relative standard deviation of less than 3.0%. The proposed method was applied successfully to the determination of lysozyme in human tear and saliva samples, and the recovery was from 92.0% to 105.7%.
収録刊行物
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- Analytical Sciences
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Analytical Sciences 19 (3), 347-352, 2003
社団法人 日本分析化学会
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詳細情報 詳細情報について
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- CRID
- 1390001204258608128
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- NII論文ID
- 130004440722
- 50000769988
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- NII書誌ID
- AA10500785
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- ISSN
- 13482246
- 09106340
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- NDL書誌ID
- 6475913
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- PubMed
- 12675337
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- Web Site
- http://id.ndl.go.jp/bib/6475913
- https://ndlsearch.ndl.go.jp/books/R000000004-I6475913
- https://link.springer.com/content/pdf/10.2116/analsci.19.347.pdf
- https://link.springer.com/article/10.2116/analsci.19.347/fulltext.html
- http://www.jstage.jst.go.jp/article/analsci/19/3/19_3_347/_pdf
- https://search.jamas.or.jp/link/ui/2003204374
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
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- PubMed
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