In-Gel Staining of Proteins in Native Poly Acryl Amide Gel Electrophoresis Using Tetrakis(4-sulfonato phenyl)porphyrin

DIVAKAR Kalivarathan, SUJATHA Vijayan, BARATH Sridhar, SRINATH Krishnamurthy, GAUTAM Pennathur

doi:10.2116/analsci.27.101

In-Gel Staining of Proteins in Native Poly Acryl Amide Gel Electrophoresis Using Tetrakis(4-sulfonato phenyl)porphyrin

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DIVAKAR Kalivarathan

Centre for Biotechnology, Anna University Chennai
SUJATHA Vijayan

Centre for Biotechnology, Anna University Chennai
BARATH Sridhar

Centre for Biotechnology, Anna University Chennai
SRINATH Krishnamurthy

Centre for Biotechnology, Anna University Chennai
GAUTAM Pennathur

Centre for Biotechnology, Anna University Chennai

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Protein identification in polyacrylamide gel electrophoresis (PAGE) requires post-electrophoretic steps like fixing, staining and destaining of the gel, which are time-consuming and cumbersome. We have developed a method for direct visualization of protein bands in PAGE using tetrakis(4-sulfonato phenyl)porphyrin (TPPS) as a dye without the need for any post electrophoretic steps, where separation and recovery of enzymes become much easier for further analysis. Activity staining was done to prove that the biochemical activity of the enzymes was preserved after electrophoresis.