Optimization of Peptide Density on Microarray Surface for Quantitative Phosphoproteomics
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- SHIMOMURA Takashi
- Department of Applied Chemistry, Kyushu University
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- HAN Xiaoming
- Department of Applied Chemistry, Kyushu University
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- HATA Akito
- Department of Applied Chemistry, Kyushu University
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- NIIDOME Takuro
- Department of Applied Chemistry, Kyushu University Center for Future Chemistry, Kyushu University
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- MORI Takeshi
- Department of Applied Chemistry, Kyushu University Center for Future Chemistry, Kyushu University
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- KATAYAMA Yoshiki
- Department of Applied Chemistry, Kyushu University Center for Future Chemistry, Kyushu University
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Abstract
Procedures to determine the density of peptides immobilized on a glass surface for the quantitative detection of phosphorylated peptides for phosphoproteomic applications of peptide microarrays are described. Two kinds of representative fluorescent probe molecules, anti-phosphotyrosine antibody (AB) and phos-tag (PT), were examined to compare their ability for the quantitative detection of phosphorylated peptides. PT is a metal complex with a binding specificity to phosphorylated amino acids, and is much smaller in size than AB. Thus, PT is quantitatively bound to the phosphorylated peptides, even at a high immobilization density without steric hindrance, making them highly suited for future microarrays requiring smaller sized peptide spots for much higher throughput.
Journal
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- Analytical Sciences
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Analytical Sciences 27 (1), 13-17, 2011
The Japan Society for Analytical Chemistry
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Keywords
Details 詳細情報について
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- CRID
- 1390001204258973184
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- NII Article ID
- 10027739412
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- NII Book ID
- AA10500785
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- ISSN
- 13482246
- 09106340
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- NDL BIB ID
- 10932866
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
- KAKEN
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- Abstract License Flag
- Disallowed