Pharmacokinetics of Propranolol Hydrochlorid in Human Urine by Capillary Electrophoresis Coupled with Electrochemiluminescence

DENG Biyang, YIN Huihui, LIU Yang, NING Xi

doi:10.2116/analsci.27.55

Pharmacokinetics of Propranolol Hydrochlorid in Human Urine by Capillary Electrophoresis Coupled with Electrochemiluminescence

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DENG Biyang

Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources (Ministry of Education of China), College of Chemistry and Chemical Engineering, Guangxi Normal University
YIN Huihui

Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources (Ministry of Education of China), College of Chemistry and Chemical Engineering, Guangxi Normal University
LIU Yang

Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources (Ministry of Education of China), College of Chemistry and Chemical Engineering, Guangxi Normal University
NING Xi

Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources (Ministry of Education of China), College of Chemistry and Chemical Engineering, Guangxi Normal University

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抄録

A novel method for the determination of propranolol hydrochlorid (PRO) in human urine was developed using capillary electrophoresis coupled with electrochemiluminescence detection (CE-ECL). The parameters that affected the separation and detection were optimized. Under the optimal conditions, the linear range for PRO was from 0.003 to 2 μg/mL (r² = 0.9993), and the detection limit was 1.3 ng/mL (S/N = 3). The method was successfully applied to the study of the pharmacokinetics of PRO in human urine. The relative standard deviations of ECL intensity and migration time were 2.6 and 2.1%, respectively (1.0 μg/mL PRO, n = 6). The recovery was between 96.71 and 97.30%. The peak excretion rate in urine was observed during the 0.5 – 1 h after oral administration of a 10-mg PRO tablet and the urinary excretion ratio of PRO was 13.6% within 12 h. The method was simple, rapid, economical and sensitive, and may improve the detection of PRO as a doping agent in sports.