Ultrasensitive Biosensor for Detection of Organophosphorus Pesticides Based on a Macrocycle Complex/Carbon Nanotubes Composite and 1-Methyl-3-octylimidazolium Tetrafluoroborate as Binder Compound
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- PEREIRA Neuma das Mercês
- Departamento de Química, UFVJM
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- OLIVEIRA Fernando Mota de
- Departamento de Química, UFVJM
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- PEREIRA Nara Rúbia
- Instituto de Ciências Exatas e Biológicas, UFOP
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- VERLY Rodrigo Moreira
- Departamento de Química, UFVJM
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- SOUTO Dênio Emanuel Pires
- Instituto de Química, UNICAMP
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- KUBOTA Lauro Tatsuo
- Instituto de Química, UNICAMP
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- TANAKA Auro Atsushi
- Departamento de Química, UFMA
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- DAMOS Flavio Santos
- Departamento de Química, UFMA
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- LUZ Rita de Cássia Silva
- Departamento de Química, UFMA
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This work describes the highly sensitive detection of organophosphorus pesticides employing the cobalt(II) 4,4,4,4-tetrasulfo-phthalocyanine (CoTSPc) macrocycle complex, carbon nanotubes (CNT), and 1-methyl-3-octylimidazolium tetrafluoroborate (OMIM[BF4]). The technique is based on enzyme acetylcholinesterase (AChE) inhibition. The composite was characterized by scanning electron microscopy (SEM), Fourier transform infrared (FT-IR) spectroscopy, and amperometry. The AChE was immobilized on the composite electrode surface by cross-linking with glutaraldehyde and chitosan. The synergistic action of the CoTSPc/CNT/OMIM[BF4] composite showed excellent electrocatalytic activity, with a low applied potential for the amperometric detection of thiocholine (TCh) at 0.0 V vs. Ag/AgCl. The calculated catalytic rate constant, kcat, was 3.67 × 103 mol−1 L s−1. Under the optimum conditions, the inhibition rates of these pesticides were proportional to their concentrations in the ranges of 1.0 pmol L−1 to 1.0 nmol L−1 (fenitrothion), 2.0 pmol L−1 to 8.0 nmol L−1 (dichlorvos), and 16 pmol L−1 to 5.0 nmol L−1 (malathion).
収録刊行物
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- Analytical Sciences
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Analytical Sciences 31 (1), 29-35, 2015
社団法人 日本分析化学会
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詳細情報 詳細情報について
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- CRID
- 1390001204259772032
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- NII論文ID
- 130004827443
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- NII書誌ID
- AA10500785
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- ISSN
- 13482246
- 09106340
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- NDL書誌ID
- 026048544
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- PubMed
- 25792271
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可