Quantitative Live Single-cell Mass Spectrometry with Spatial Evaluation by Three-Dimensional Holographic and Tomographic Laser Microscopy
-
- ALI Ahmed
- Quantitative Biology Center (QBiC), RIKEN Faculty of Pharmacy, Misr International University
-
- ABOULEILA Yasmine
- Quantitative Biology Center (QBiC), RIKEN Faculty of Pharmacy, Misr International University
-
- AMER Sara
- Quantitative Biology Center (QBiC), RIKEN Faculty of Pharmacy, Misr International University
-
- FURUSHIMA Rie
- Quantitative Biology Center (QBiC), RIKEN
-
- EMARA Samy
- Faculty of Pharmacy, Misr International University
-
- EQUIS Sebastien
- Nanolive SA
-
- COTTE Yann
- Nanolive SA
-
- MASUJIMA Tsutomu
- Quantitative Biology Center (QBiC), RIKEN
この論文をさがす
抄録
The locations and volumes of the contents of a single HepG2 cell were visualized under three-dimensional (3D) holographic and tomographic (HT) laser microscopy, colored by refractive index, not staining. After trapping the specific area of a target cell in a nanospray tip, quantification was performed by live single-cell mass spectrometry. Comparison of the HepG2 cells’ before and after 3D-HT images allowed the inference of the precise volume and original location of the trapped cell contents. The total amount of a trapped molecule was estimated. The images also revealed morphological changes in the cell structure caused by the manipulation.
収録刊行物
-
- Analytical Sciences
-
Analytical Sciences 32 (2), 125-127, 2016
社団法人 日本分析化学会
- Tweet
キーワード
詳細情報 詳細情報について
-
- CRID
- 1390001204259938048
-
- NII論文ID
- 130005125630
-
- NII書誌ID
- AA10500785
-
- ISSN
- 13482246
- 09106340
-
- NDL書誌ID
- 027107192
-
- PubMed
- 26860553
-
- Web Site
- http://id.ndl.go.jp/bib/027107192
- https://ndlsearch.ndl.go.jp/books/R000000004-I027107192
- https://link.springer.com/content/pdf/10.2116/analsci.32.125.pdf
- https://link.springer.com/article/10.2116/analsci.32.125/fulltext.html
- https://www.jstage.jst.go.jp/article/analsci/32/2/32_125/_pdf
- https://search.jamas.or.jp/link/ui/2016293171
-
- 本文言語コード
- en
-
- データソース種別
-
- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
-
- 抄録ライセンスフラグ
- 使用不可
