Photochemical Immobilization of Cells onto a Glass Substrate for in situ DNA Analysis

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  • SASAKI Naoki
    Department of Chemical and Biological Sciences, Faculty of Science, Japan Women’s University
  • ISU Anri
    Department of Chemical and Biological Sciences, Faculty of Science, Japan Women’s University
  • ISHII Reina
    Department of Chemical and Biological Sciences, Faculty of Science, Japan Women’s University
  • SATO Kae
    Department of Chemical and Biological Sciences, Faculty of Science, Japan Women’s University

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タイトル別名
  • Photochemical Immobilization of Cells onto a Glass Substrate for <i>in situ</i> DNA Analysis

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抄録

A simple and robust method to immobilize cells onto a glass substrate is presented. The method employs a photochemical reaction of benzophenone, which is modified on the substrate using a standard silane coupling agent, with cells. Cells were immobilized to an area irradiated with UV light from a standard light source under an inverted microscope. The dependence of immobilization on the light power intensity and irradiation time was investigated. In situ DNA analysis within the immobilized cells was demonstrated using target-primed rolling circle amplification and fluorescent detection.

収録刊行物

  • Analytical Sciences

    Analytical Sciences 28 (6), 537-539, 2012

    社団法人 日本分析化学会

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