Photochemical Immobilization of Cells onto a Glass Substrate for in situ DNA Analysis
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- SASAKI Naoki
- Department of Chemical and Biological Sciences, Faculty of Science, Japan Women’s University
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- ISU Anri
- Department of Chemical and Biological Sciences, Faculty of Science, Japan Women’s University
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- ISHII Reina
- Department of Chemical and Biological Sciences, Faculty of Science, Japan Women’s University
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- SATO Kae
- Department of Chemical and Biological Sciences, Faculty of Science, Japan Women’s University
書誌事項
- タイトル別名
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- Photochemical Immobilization of Cells onto a Glass Substrate for <i>in situ</i> DNA Analysis
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抄録
A simple and robust method to immobilize cells onto a glass substrate is presented. The method employs a photochemical reaction of benzophenone, which is modified on the substrate using a standard silane coupling agent, with cells. Cells were immobilized to an area irradiated with UV light from a standard light source under an inverted microscope. The dependence of immobilization on the light power intensity and irradiation time was investigated. In situ DNA analysis within the immobilized cells was demonstrated using target-primed rolling circle amplification and fluorescent detection.
収録刊行物
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- Analytical Sciences
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Analytical Sciences 28 (6), 537-539, 2012
社団法人 日本分析化学会
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詳細情報 詳細情報について
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- CRID
- 1390001204259956480
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- NII論文ID
- 10030757784
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- NII書誌ID
- AA10500785
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- COI
- 1:STN:280:DC%2BC38jksVGquw%3D%3D
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- ISSN
- 13482246
- 09106340
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- NDL書誌ID
- 023689163
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- PubMed
- 22729038
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可