Characterization of Protein-like Fluorophores Released from Lake Phytoplankton on the Basis of Fractionation and Electrophoresis
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- YAMADA Etsu
- Center for Environmental Science, Kyoto Institute of Technology Department of Chemistry and Material Technology, Kyoto Institute of Technology
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- HIROTA Takaaki
- Department of Chemistry and Material Technology, Kyoto Institute of Technology
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- HATORI Naoko
- Department of Chemistry and Material Technology, Kyoto Institute of Technology
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- KITAO Yuki
- Department of Chemistry and Material Technology, Kyoto Institute of Technology
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- FUSE Yasuro
- Center for Environmental Science, Kyoto Institute of Technology
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- AOKI Shinichi
- Lake Biwa Environmental Research Institute (LBERI)
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- KARATANI Hajime
- Department of Biomolecular Engineering, Kyoto Institute of Technology
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- MATSUNAGA Toshiro
- National Agricultural Research Center, National Agriculture and Food Research Organization
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Abstract
Three kinds of lake plankton were cultivated, and the properties of protein-like fluorophores released from the plankton were characterized using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The results were compared with those by gel chromatography with a fluorescence detector and three-dimensional excitation-emission matrix (3-DEEM). The concentrated protein-like fluorophores of algal dissolved organic matter (DOM) were successfully separated from the fulvic-like fluorophores, and analyzed using SDS-PAGE. SDS-PAGE analysis revealed that the protein-like fluorescence DOM released from Microcystis aeruginosa consisted of proteins with molecular weights of 17, 37, 50, 75, 150 kDa, and greater than 250 kDa. The results of SDS-PAGE were consistent with those of gel chromatography. Those substances with molecular weights greater than 250 kDa may be a polysaccharide-peptide complex, called peptidoglycan, which is a component of bacterial cell walls. The molecular weights of protein-like fluorescence DOM from Staurastrum dorsidentiferum were determined to be 37 and 50 kDa. For Cryptomonas ovata, its DOM was found to be composed of substances with molecular weights of between 10 and 150 kDa. The results by high-performance size exclusion chromatography with chemiluminescent nitrogen detection (HPSEC/CLND) analysis suggest that the protein-like fluorophores from the plankton might be composed of substances containing organic nitrogen.
Journal
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- Analytical Sciences
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Analytical Sciences 28 (6), 595-600, 2012
The Japan Society for Analytical Chemistry
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Details 詳細情報について
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- CRID
- 1390001204259966720
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- NII Article ID
- 10030758052
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- NII Book ID
- AA10500785
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- COI
- 1:STN:280:DC%2BC38jksVKjtQ%3D%3D
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- ISSN
- 13482246
- 09106340
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- NDL BIB ID
- 023689206
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- PubMed
- 22729046
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
- KAKEN
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- Abstract License Flag
- Disallowed