Detection of Enrofloxacin and Its Metabolite Ciprofloxacin Using Gold Nanoparticles and Laser Desorption/Ionization Time-of-Flight Mass Spectrometry
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- HA Mi-Sun
- Department of Bioscience and Biotechnology, Konkuk University
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- SEO Hyunjung
- Department of Bioscience and Biotechnology, Bio/Molecular Informatics Center, Konkuk University
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- BAE Dong-Ho
- Department of Bioscience and Biotechnology, Konkuk University
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- YEO Woon-Seok
- Department of Bioscience and Biotechnology, Bio/Molecular Informatics Center, Konkuk University
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Abstract
This paper describes a new, simple, and sensitive method for detecting two fluoroquinolones: enrofloxacin and its metabolite ciprofloxacin, which are widely used as drugs for humans and animals. We utilized gold nanoparticles (AuNPs) and laser desorption/ionization time-of-flight (LDI-TOF) mass spectrometry (MS) with a matrix-free format. An antibody for the drug was immobilized on a chip based on self-assembled monolayers (SAMs) on gold, and AuNPs were decorated with the drug along with a large excess of small molecules, called amplification tags (Am-tags). In this strategy, target drugs in solution bound to the antibody on the chip compete with the AuNP-immobilized drugs. The presence of targets was verified by the amplified LDI-TOF MS signals of Am-tags on AuNPs.
Journal
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- Analytical Sciences
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Analytical Sciences 30 (4), 451-455, 2014
The Japan Society for Analytical Chemistry
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Details 詳細情報について
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- CRID
- 1390001204260088576
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- NII Article ID
- 130004442272
- 40020036828
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- NII Book ID
- AA10500785
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- COI
- 1:STN:280:DC%2BC2cnitlWlug%3D%3D
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- ISSN
- 13482246
- 09106340
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- NDL BIB ID
- 025389794
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- PubMed
- 24717653
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed