Quantification of Proteins by Measuring the Sulfur Content of Their Constituent Peptides by Means of Nano HPLC-ICPMS
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- SUZUKI Yoshinari
- Faculty of Science and Engineering, Department of Applied Chemistry, Chuo University
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- NOBUSAWA Ayumi
- Faculty of Science and Engineering, Department of Applied Chemistry, Chuo University
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- FURUTA Naoki
- Faculty of Science and Engineering, Department of Applied Chemistry, Chuo University
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Abstract
The sulfur (S) concentrations of three peptides were determined by using nano HPLC-ICPMS under a flow of O2 in an octapole reaction cell, and the determined values showed a good agreement with theoretical values. This method was then applied to trypsin-digested peptides from human albumin for protein quantification. Assigning of the number of S atoms in each peptide/peak and the tryptic digestion efficiency were important for protein quantification. The number of S atoms in each peptide/peak was assigned by using verification scores that gave the lowest standard deviation of the peptide S concentration and the highest S recovery. The peptide concentrations were calculated as the ratio of the S concentration/the number of S atoms in the peptide/peak. The tryptic digestion efficiency was calculated as the sum of the S concentration in the mono-peptides divided by the total S concentration in a native polyacrylamide gel electrophoresis (PAGE) band before tryptic digestion. Our result indicates that a protein can be quantified through peptide quantification, after taking into account the tryptic digestion efficiency, via S quantification using ICPMS.
Journal
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- Analytical Sciences
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Analytical Sciences 30 (5), 551-559, 2014
The Japan Society for Analytical Chemistry
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Details 詳細情報について
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- CRID
- 1390001204260236672
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- NII Article ID
- 130004442287
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- NII Book ID
- AA10500785
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- COI
- 1:STN:280:DC%2BC2cjhslelsQ%3D%3D
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- ISSN
- 13482246
- 09106340
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- NDL BIB ID
- 025440674
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- PubMed
- 24813953
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- Web Site
- http://id.ndl.go.jp/bib/025440674
- https://ndlsearch.ndl.go.jp/books/R000000004-I025440674
- https://link.springer.com/content/pdf/10.2116/analsci.30.551.pdf
- https://link.springer.com/article/10.2116/analsci.30.551/fulltext.html
- https://www.jstage.jst.go.jp/article/analsci/30/5/30_551/_pdf
- https://search.jamas.or.jp/link/ui/2015033226
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
- KAKEN
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- Abstract License Flag
- Disallowed