On-chip FRET Graphene Oxide Aptasensor: Quantitative Evaluation of Enhanced Sensitivity by Aptamer with a Double-stranded DNA Spacer
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- UENO Yuko
- NTT Basic Research Laboratories, NTT Corp.
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- FURUKAWA Kazuaki
- NTT Basic Research Laboratories, NTT Corp.
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- TIN Andrew
- NTT Basic Research Laboratories, NTT Corp.
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- HIBINO Hiroki
- NTT Basic Research Laboratories, NTT Corp.
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Abstract
We propose a molecular design for a biomolecular probe to realize an on-chip graphene oxide (GO) aptasensor with enhanced sensitivity. Here, GO works as an excellent acceptor for fluorescence resonance energy transfer. We inserted a rigid double-stranded DNA as a spacer between the GO surface and the aptamer sequence to extend the distance between a fluorescence dye and the GO surface during molecular recognition. We examined the dependence of the sensitivity on the length of the spacer quantitatively by using a 2×2 linear-array aptasensor. We used the modified aptamer with 10 and 30 base pair (bp) double-stranded DNA spacers. The signal with a 30bp-spacer was about twice as strong that with a 10bp-spacer as regards both thrombin and prostate specific antigen detections. The improvement in the sensitivity was supported by a model calculation that estimated the effect of spacer length on fluorescence recovery efficiency.
Journal
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- Analytical Sciences
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Analytical Sciences 31 (9), 875-879, 2015
The Japan Society for Analytical Chemistry
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Keywords
Details 詳細情報について
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- CRID
- 1390001204260288768
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- NII Article ID
- 130005097923
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- NII Book ID
- AA10500785
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- ISSN
- 13482246
- 09106340
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- NDL BIB ID
- 026735619
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- PubMed
- 26353952
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- Web Site
- http://id.ndl.go.jp/bib/026735619
- https://ndlsearch.ndl.go.jp/books/R000000004-I026735619
- https://link.springer.com/content/pdf/10.2116/analsci.31.875.pdf
- https://link.springer.com/article/10.2116/analsci.31.875/fulltext.html
- https://www.jstage.jst.go.jp/article/analsci/31/9/31_875/_pdf
- https://search.jamas.or.jp/link/ui/2016154594
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
- KAKEN
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- Abstract License Flag
- Disallowed