Encapsulation of PEG-modified Myoglobin in Hydrophobic Mesoporous Silica as Studied by Optical Waveguide Spectroscopy

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  • ARAFUNE Hiroyuki
    Department of Chemistry, Graduate School of Science, Tohoku University
  • YAMAGUCHI Akira
    College of Science and Frontier Research Center for Applied Atomic Sciences, Ibaraki University
  • HOTTA Kazuhiro
    Department of Chemistry, Graduate School of Science, Tohoku University
  • ITOH Tetsuji
    Research Center for Compact Chemical System, National Institute of Advanced Industrial Science and Technology (AIST)
  • TERAMAE Norio
    Department of Chemistry, Graduate School of Science, Tohoku University

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抄録

The purpose of this study is to apply optical waveguide (OWG) spectroscopy to characterize the encapsulation behavior of enzymes modified with polyethylene glycol (PEG), i.e. pegylation, in a hydrophobic mesoporous silica film. For that purpose, pegylated myoglobin (PEG-Mb) was introduced into the silica mesopores modified with octadecylsilyl (ODS) groups and studied by OWG spectroscopy. OWG spectroscopy confirmed that the hydrophobic interaction between the PEG group and the surface ODS group promoted the encapsulation of PEG-Mb into the hydrophobic silica mesopores. The surface density of ODS affected the adsorbed amount of PEG-Mb and the higher surface density of the ODS group resulted in the suppression of adsorption and diffusion of PEG-Mb inside the pore. Since the desorption rate of PEG-Mb was found to be much slower than the adsorption rate, the pegylation of an enzyme could be effective for the enzyme encapsulation into the hydrophobic mesoporous silica host.

収録刊行物

  • Analytical Sciences

    Analytical Sciences 29 (2), 187-192, 2013

    社団法人 日本分析化学会

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