Label-free, Water-soluble Fluorescent Peptide Probe for a Sensitive and Selective Determination of Copper Ions

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  • YANG Tao
    National Engineering Laboratory for Rice and By-products Further Processing, Central South University of Forestry and Technology Department of Food Science and Technology, Central South University of Forestry and Technology
  • ZHU Fang
    National Engineering Laboratory for Rice and By-products Further Processing, Central South University of Forestry and Technology Department of Food Science and Technology, Central South University of Forestry and Technology
  • ZHOU Tianxiao
    National Engineering Laboratory for Rice and By-products Further Processing, Central South University of Forestry and Technology Department of Food Science and Technology, Central South University of Forestry and Technology
  • CAO Jianzhong
    National Engineering Laboratory for Rice and By-products Further Processing, Central South University of Forestry and Technology Department of Food Science and Technology, Central South University of Forestry and Technology
  • XIE Yibo
    National Engineering Laboratory for Rice and By-products Further Processing, Central South University of Forestry and Technology Department of Food Science and Technology, Central South University of Forestry and Technology
  • ZHANG Meiyi
    National Engineering Laboratory for Rice and By-products Further Processing, Central South University of Forestry and Technology Department of Food Science and Technology, Central South University of Forestry and Technology
  • WANG Yan
    National Engineering Laboratory for Rice and By-products Further Processing, Central South University of Forestry and Technology Department of Food Science and Technology, Central South University of Forestry and Technology
  • CAO Dong-Sheng
    School of Pharmaceutical Sciences, Central South University
  • LIN Qinlu
    National Engineering Laboratory for Rice and By-products Further Processing, Central South University of Forestry and Technology Department of Food Science and Technology, Central South University of Forestry and Technology
  • ZHANG Lin
    National Engineering Laboratory for Rice and By-products Further Processing, Central South University of Forestry and Technology Department of Food Science and Technology, Central South University of Forestry and Technology

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抄録

This study aimed to develop a label-free, sensitive, selective, and environment-friendly fluorescent peptide probe His-His-Trp-His (HHWH) for determining the concentration of copper ion (Cu2+) in aqueous solutions. The results demonstrated that the designed HHWH has a high selectivity and sensitivity for monitoring the concentration of free Cu2+ via quenching of the probe fluorescence upon a binding of Cu2+. The fluorescence intensity of the HHWH had a linear relationship with the concentration of Cu2+ between 10 nM and 10 μM, and the detection limit was 8 nM. Furthermore, HHWH could be regenerated with sulfide ions at least five times. The concentrations of Cu2+ in three different real water samples were detected using this probe, and the results were consistent with the one detected using an atomic absorption spectrometer. Thus, HHWH can be used as an accurate and feasible fluorescent peptide probe for detecting Cu2+ in aqueous solutions.

収録刊行物

  • Analytical Sciences

    Analytical Sciences 33 (2), 191-196, 2017

    社団法人 日本分析化学会

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