Label-free, Water-soluble Fluorescent Peptide Probe for a Sensitive and Selective Determination of Copper Ions
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- YANG Tao
- National Engineering Laboratory for Rice and By-products Further Processing, Central South University of Forestry and Technology Department of Food Science and Technology, Central South University of Forestry and Technology
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- ZHU Fang
- National Engineering Laboratory for Rice and By-products Further Processing, Central South University of Forestry and Technology Department of Food Science and Technology, Central South University of Forestry and Technology
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- ZHOU Tianxiao
- National Engineering Laboratory for Rice and By-products Further Processing, Central South University of Forestry and Technology Department of Food Science and Technology, Central South University of Forestry and Technology
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- CAO Jianzhong
- National Engineering Laboratory for Rice and By-products Further Processing, Central South University of Forestry and Technology Department of Food Science and Technology, Central South University of Forestry and Technology
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- XIE Yibo
- National Engineering Laboratory for Rice and By-products Further Processing, Central South University of Forestry and Technology Department of Food Science and Technology, Central South University of Forestry and Technology
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- ZHANG Meiyi
- National Engineering Laboratory for Rice and By-products Further Processing, Central South University of Forestry and Technology Department of Food Science and Technology, Central South University of Forestry and Technology
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- WANG Yan
- National Engineering Laboratory for Rice and By-products Further Processing, Central South University of Forestry and Technology Department of Food Science and Technology, Central South University of Forestry and Technology
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- CAO Dong-Sheng
- School of Pharmaceutical Sciences, Central South University
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- LIN Qinlu
- National Engineering Laboratory for Rice and By-products Further Processing, Central South University of Forestry and Technology Department of Food Science and Technology, Central South University of Forestry and Technology
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- ZHANG Lin
- National Engineering Laboratory for Rice and By-products Further Processing, Central South University of Forestry and Technology Department of Food Science and Technology, Central South University of Forestry and Technology
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抄録
This study aimed to develop a label-free, sensitive, selective, and environment-friendly fluorescent peptide probe His-His-Trp-His (HHWH) for determining the concentration of copper ion (Cu2+) in aqueous solutions. The results demonstrated that the designed HHWH has a high selectivity and sensitivity for monitoring the concentration of free Cu2+ via quenching of the probe fluorescence upon a binding of Cu2+. The fluorescence intensity of the HHWH had a linear relationship with the concentration of Cu2+ between 10 nM and 10 μM, and the detection limit was 8 nM. Furthermore, HHWH could be regenerated with sulfide ions at least five times. The concentrations of Cu2+ in three different real water samples were detected using this probe, and the results were consistent with the one detected using an atomic absorption spectrometer. Thus, HHWH can be used as an accurate and feasible fluorescent peptide probe for detecting Cu2+ in aqueous solutions.
収録刊行物
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- Analytical Sciences
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Analytical Sciences 33 (2), 191-196, 2017
社団法人 日本分析化学会
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詳細情報 詳細情報について
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- CRID
- 1390001204260751360
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- NII論文ID
- 130005316212
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- NII書誌ID
- AA10500785
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- ISSN
- 13482246
- 09106340
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- NDL書誌ID
- 027968927
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- PubMed
- 28190839
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可