Highly Sensitive Fluorescent Immunoassay of Human Immunoglobulin G Based on PbS Nanoparticles and DNAzyme
-
- HUANG FU Zhen-Zhen
- State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University
-
- HAO Li-Juan
- State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University
-
- WU Yan-Mei
- State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University
-
- QIAO Hai-Ying
- State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University
-
- YI Zi
- State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University
-
- LI Xiao-Yan
- State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University
-
- CHU Xia
- State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University
この論文をさがす
抄録
This paper reports a highly sensitive fluorescent immunoassay for the detection of human immunoglobulin G based on PbS nanoparticles and DNAzyme. A sandwich immunoassay format was performed on a microtiter plate. Goat anti-human IgG was coated onto the polystyrene microtiter plate. The human IgG analyte was first captured by the goat anti-human IgG, and then sandwiched by a goat anti-human IgG antibody labeled with PbS nanoparticles. After being dissolved with HNO3, the released Pb2+ made the substrate chain of the DNAzyme labeled with the fluorophore dissociate from the enzyme strand of the DNAzyme labeled with the quencher, which resulted in fluorescence recovery. Then, the human IgG could be detected indirectly from the fluorescent signals. Under the optimized conditions, the linear range of the developed immunosensor was from 1 ng mL−1 to 10 μg mL−1 with a detection limit of 0.8 ng mL−1. This immunosensor could be used to detect the amount of human IgG in human serum samples.
収録刊行物
-
- Analytical Sciences
-
Analytical Sciences 29 (5), 499-504, 2013
社団法人 日本分析化学会
- Tweet
キーワード
詳細情報 詳細情報について
-
- CRID
- 1390001204260771072
-
- NII論文ID
- 10031170977
-
- NII書誌ID
- AA10500785
-
- COI
- 1:STN:280:DC%2BC3snislejtg%3D%3D
-
- ISSN
- 13482246
- 09106340
-
- NDL書誌ID
- 024473595
-
- PubMed
- 23665621
-
- Web Site
- http://id.ndl.go.jp/bib/024473595
- https://ndlsearch.ndl.go.jp/books/R000000004-I024473595
- https://link.springer.com/content/pdf/10.2116/analsci.29.499.pdf
- https://link.springer.com/article/10.2116/analsci.29.499/fulltext.html
- https://www.jstage.jst.go.jp/article/analsci/29/5/29_499/_pdf
- https://search.jamas.or.jp/link/ui/2014010844
-
- 本文言語コード
- en
-
- データソース種別
-
- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
-
- 抄録ライセンスフラグ
- 使用不可