Highly Sensitive Fluorescent Immunoassay of Human Immunoglobulin G Based on PbS Nanoparticles and DNAzyme

DOI PDF Web Site Web Site Web Site ほか1件をすべて表示 一部だけ表示 参考文献69件
  • HUANG FU Zhen-Zhen
    State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University
  • HAO Li-Juan
    State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University
  • WU Yan-Mei
    State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University
  • QIAO Hai-Ying
    State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University
  • YI Zi
    State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University
  • LI Xiao-Yan
    State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University
  • CHU Xia
    State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University

この論文をさがす

抄録

This paper reports a highly sensitive fluorescent immunoassay for the detection of human immunoglobulin G based on PbS nanoparticles and DNAzyme. A sandwich immunoassay format was performed on a microtiter plate. Goat anti-human IgG was coated onto the polystyrene microtiter plate. The human IgG analyte was first captured by the goat anti-human IgG, and then sandwiched by a goat anti-human IgG antibody labeled with PbS nanoparticles. After being dissolved with HNO3, the released Pb2+ made the substrate chain of the DNAzyme labeled with the fluorophore dissociate from the enzyme strand of the DNAzyme labeled with the quencher, which resulted in fluorescence recovery. Then, the human IgG could be detected indirectly from the fluorescent signals. Under the optimized conditions, the linear range of the developed immunosensor was from 1 ng mL−1 to 10 μg mL−1 with a detection limit of 0.8 ng mL−1. This immunosensor could be used to detect the amount of human IgG in human serum samples.

収録刊行物

  • Analytical Sciences

    Analytical Sciences 29 (5), 499-504, 2013

    社団法人 日本分析化学会

参考文献 (69)*注記

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ