Reducing Acyl Migration during Purification of 2-Arachidonoylglycerol from Biological Samples before Gas Chromatography Mass Spectrometry Analysis

HIGUCHI Sei, IRIE Keiichi, NAKANO Takafumi, SAKAMOTO Yuya, AKITAKE Yoshiharu, ARAKI Maiko, OHJI Makiko, FURUTA Riyo, KATSUKI Mai, YAMAGUCHI Ryuji, MATSUYAMA Kiyoshi, MISHIMA Kenji, MISHIMA Kenichi, IWASAKI Katsunori, FUJIWARA Michihiro

doi:10.2116/analsci.26.1199

Reducing Acyl Migration during Purification of 2-Arachidonoylglycerol from Biological Samples before Gas Chromatography Mass Spectrometry Analysis

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HIGUCHI Sei

Department of Neuropharmacology, Faculty of Pharmaceutical Sciences, Fukuoka University
IRIE Keiichi

Department of Neuropharmacology, Faculty of Pharmaceutical Sciences, Fukuoka University Advanced Materials Institute
NAKANO Takafumi

Department of Neuropharmacology, Faculty of Pharmaceutical Sciences, Fukuoka University
SAKAMOTO Yuya

Department of Neuropharmacology, Faculty of Pharmaceutical Sciences, Fukuoka University
AKITAKE Yoshiharu

Department of Neuropharmacology, Faculty of Pharmaceutical Sciences, Fukuoka University Advanced Materials Institute
ARAKI Maiko

Department of Neuropharmacology, Faculty of Pharmaceutical Sciences, Fukuoka University
OHJI Makiko

Department of Neuropharmacology, Faculty of Pharmaceutical Sciences, Fukuoka University
FURUTA Riyo

Department of Neuropharmacology, Faculty of Pharmaceutical Sciences, Fukuoka University
KATSUKI Mai

Department of Neuropharmacology, Faculty of Pharmaceutical Sciences, Fukuoka University
YAMAGUCHI Ryuji

Department of Neuropharmacology, Faculty of Pharmaceutical Sciences, Fukuoka University
MATSUYAMA Kiyoshi

Department of Chemical Engineering, Faculty of Engineering, Fukuoka University
MISHIMA Kenji

Department of Chemical Engineering, Faculty of Engineering, Fukuoka University
MISHIMA Kenichi

Department of Neuropharmacology, Faculty of Pharmaceutical Sciences, Fukuoka University Advanced Materials Institute
IWASAKI Katsunori

Department of Neuropharmacology, Faculty of Pharmaceutical Sciences, Fukuoka University Advanced Materials Institute
FUJIWARA Michihiro

Department of Neuropharmacology, Faculty of Pharmaceutical Sciences, Fukuoka University

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抄録

Endocannabinoid 2-arachidonoylglycerol (2-AG) regulates several important physiological processes in the brain. 2-AG is commonly quantified by gas chromatography mass spectrometry after an initial purification step. The most precise and rapid purification utilizes C₁₈ solid-phase extraction, but quantification problems can arise with acyl migration from 2-AG to 1-arachidonoylglycerol. We found that extraction with methanol promoted this migration, but acetone and diethyl ether (Et₂O) did not. Acetone and Et₂O were used to develop a purification method for the direct determination of 2-AG.