Trypsin-Stabilized Fluorescent Gold Nanocluster for Sensitive and Selective Hg2+ Detection

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  • KAWASAKI Hideya
    Department of Chemistry and Materials Engineering, Faculty of Chemistry, Materials and Bioengineering, Kansai University
  • YOSHIMURA Kouta
    Department of Chemistry and Materials Engineering, Faculty of Chemistry, Materials and Bioengineering, Kansai University
  • HAMAGUCHI Kenji
    Department of Chemistry and Materials Engineering, Faculty of Chemistry, Materials and Bioengineering, Kansai University
  • ARAKAWA Ryuichi
    Department of Chemistry and Materials Engineering, Faculty of Chemistry, Materials and Bioengineering, Kansai University

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We report on trypsin-stabilized fluorescent gold nanoclusters (Au NCs) for the sensitive and selective detection of Hg2+ ions. The Au NCs have an average size of 1 nm and show a red emission at 645 nm. The photostable properties of the trypsin-stabilized Au NCs were examined, and their photochemical stability was found to be similar to that of CdSe quantum dots. The fluorescence was particularly quenched by Hg2+, and therefore the Au NCs can be used as fluorescent sensors for sensitive and selective Hg2+ detection to a detection limit of 50 ± 10 nM and the quantitative detection of Hg2+ in wide and low concentration range of 50 – 600 nM.

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  • Analytical Sciences

    Analytical Sciences 27 (6), 591-, 2011

    社団法人 日本分析化学会

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