DNA Analysis Based on the Local Structural Disruption to the Duplexes Carrying a Luminous Lanthanide Complex
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- IHARA Toshihiro
- Department of Applied Chemistry and Biochemistry, Graduate School of Science and Technology, Kumamoto University
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- KITAMURA Yusuke
- Department of Applied Chemistry and Biochemistry, Graduate School of Science and Technology, Kumamoto University
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- TSUJIMURA Yusuke
- Department of Applied Chemistry and Biochemistry, Graduate School of Science and Technology, Kumamoto University
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- JYO Akinori
- Department of Applied Chemistry and Biochemistry, Graduate School of Science and Technology, Kumamoto University
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抄録
The two DNA conjugates (split probes) carrying a metal chelator form an integrated luminous lanthanide (Ln3+: Tb3+ or Eu3+) complex on the complementary template DNA (target). The luminous property of this Ln3+ complex has been used for DNA assay. The intensity of the luminescence was affected by the local structural disruption caused by one-base mispairing around the complex. Among the mispairings systematically introduced around the Ln3+ center, vicinal mispairings to the center decreased the emission intensity more. This would be a novel nucleobase-discriminating principle, in which the split probes bind the target tightly, yet still retain sequence selectivity.
収録刊行物
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- Analytical Sciences
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Analytical Sciences 27 (6), 585-, 2011
社団法人 日本分析化学会
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詳細情報 詳細情報について
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- CRID
- 1390001204260923008
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- NII論文ID
- 10028289066
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- NII書誌ID
- AA10500785
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- COI
- 1:STN:280:DC%2BC3MrpvVGltw%3D%3D
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- ISSN
- 13482246
- 09106340
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- NDL書誌ID
- 11106236
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- PubMed
- 21666354
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
- KAKEN
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- 抄録ライセンスフラグ
- 使用不可