DNA Analysis Based on the Local Structural Disruption to the Duplexes Carrying a Luminous Lanthanide Complex

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  • IHARA Toshihiro
    Department of Applied Chemistry and Biochemistry, Graduate School of Science and Technology, Kumamoto University
  • KITAMURA Yusuke
    Department of Applied Chemistry and Biochemistry, Graduate School of Science and Technology, Kumamoto University
  • TSUJIMURA Yusuke
    Department of Applied Chemistry and Biochemistry, Graduate School of Science and Technology, Kumamoto University
  • JYO Akinori
    Department of Applied Chemistry and Biochemistry, Graduate School of Science and Technology, Kumamoto University

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抄録

The two DNA conjugates (split probes) carrying a metal chelator form an integrated luminous lanthanide (Ln3+: Tb3+ or Eu3+) complex on the complementary template DNA (target). The luminous property of this Ln3+ complex has been used for DNA assay. The intensity of the luminescence was affected by the local structural disruption caused by one-base mispairing around the complex. Among the mispairings systematically introduced around the Ln3+ center, vicinal mispairings to the center decreased the emission intensity more. This would be a novel nucleobase-discriminating principle, in which the split probes bind the target tightly, yet still retain sequence selectivity.

収録刊行物

  • Analytical Sciences

    Analytical Sciences 27 (6), 585-, 2011

    社団法人 日本分析化学会

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