Development of a Bioluminescent Bacteria Sheet for the Measurement of Oxygen Concentration

SASAKI Satoshi, MOCHIZUKI Mika, IGARASHI Makoto

doi:10.2116/analsci.29.267

Development of a Bioluminescent Bacteria Sheet for the Measurement of Oxygen Concentration

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SASAKI Satoshi

School of Bioscience and Biotechnology, Tokyo University of Technology
MOCHIZUKI Mika

School of Bioscience and Biotechnology, Tokyo University of Technology
IGARASHI Makoto

School of Bioscience and Biotechnology, Tokyo University of Technology

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The aim of this study was the preparation and characterization of a bioluminescence bacteria sheet in view of the fabrication of an oxygen marker inside a biofilm. Photobacterium kishitanii sheets were prepared using sodium alginate, and a biofilm of Pseudomonas aeruginosa was dropped over it. The relationship between the dissolved oxygen (DO) concentration in the biofilm and luminescence from the sheet was examined. As a conclusion, the alginate-gel film method was effective in fabricating a DO-monitoring thin film.