A Simplified Micropatterning Method for Straight-line Neurite Extension of Cultured Hippocampal Neurons

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  • SUZUKI Ikuro
    Graduate School of Bionics, Tokyo University of Technology
  • NAKAMURA Kosuke
    School of Bioscience and Biotechnology, Tokyo University of Technology
  • ODAWARA Aoi
    Graduate School of Bionics, Tokyo University of Technology
  • ALHEBSHI Amani
    Graduate School of Bionics, Tokyo University of Technology
  • GOTOH Masao
    Graduate School of Bionics, Tokyo University of Technology

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We report a simplified micropatterning method for the straight-line extension of the neurites of cultured neurons. We prepared a poly-D-lysine (PDL)-patterned surface using a polydimethylsiloxane microfluidic stamp. Hippocampal neurons were cultured on the PDL-bound substrate with the stamp removed, allowing for conventional cell seeding and detailed optical observation without fluorescent label. Cultured neurons elongated neurites along straight lines at the single-cell level and displayed spontaneous firing as detected by time-lapse imaging and Ca2+ imaging.

収録刊行物

  • Analytical Sciences

    Analytical Sciences 29 (2), 263-266, 2013

    社団法人 日本分析化学会

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