Pretreatment Method for Immunoassay of Polychlorinated Biphenyls in Transformer Oil Using Multilayer Capillary Column and Microfluidic Liquid–Liquid Partitioning

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  • AOTA Arata
    Environmental Science Research Laboratory, Central Research Institute of Electric Power Industry
  • DATE Yasumoto
    Environmental Science Research Laboratory, Central Research Institute of Electric Power Industry Graduate School of Environmental Studies, Tohoku University
  • TERAKADO Shingo
    Environmental Science Research Laboratory, Central Research Institute of Electric Power Industry
  • OHMURA Naoya
    Environmental Science Research Laboratory, Central Research Institute of Electric Power Industry

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Polychlorinated biphenyls (PCBs) are persistent organic pollutants that are present in the insulating oil inside a large number of transformers. To aid in eliminating PCB-contaminated transformers, PCBs in oil need to be measured using a rapid and cost-effective analytical method. We previously reported a pretreatment method for the immunoassay of PCBs in oil using a large-scale multilayer column and a microchip with multiple microrecesses, which permitted concentrated solvent extraction. In this paper, we report on a more rapid and facile pretreatment method, without an evaporation process, by improving the column and the microchip. In a miniaturized column, the decomposition and separation of oil were completed in 2 min. PCBs can be eluted from the capillary column at concentrations seven-times higher than those from the previous column. The total volume of the microrecesses was increased by improving the microrecess structure, the enabling extraction of four-times the amount of PCBs achieved with the previous system. By interfacing the capillary column with the improved microchip, PCBs in the eluate from the column were extracted into dimethyl sulfoxide in microrecesses with high enrichment and without the need for evaporation. Pretreatment was completed within 20 min. The pretreated oil was analyzed using a flow-based kinetic exclusion immunoassay. The limit of detection of PCBs in oil was 0.15 mg kg−1, which satisfies the criterion set in Japan of 0.5 mg kg−1.

収録刊行物

  • Analytical Sciences

    Analytical Sciences 29 (4), 393-399, 2013

    社団法人 日本分析化学会

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