Rapid Enzyme-linked Immunosorbent Assays for Diagnosis of Diabetes in a Compact Disc-shaped Microfluidic Device

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  • FURUTANI Shunsuke
    Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST) Advanced Photonics and Biosensing Open Innovation Laboratory (PhotoBIO-OIL), National Institute of AIST
  • NISHIO Keiko
    Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST)
  • NARUISHI Nahoko
    Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST)
  • AKAZAWA-OGAWA Yoko
    Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST)
  • HAGIHARA Yoshihisa
    Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST)
  • YOSHIDA Yasukazu
    Health Research Institute, National Institute of AIST
  • NAGAI Hidenori
    Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST) Advanced Photonics and Biosensing Open Innovation Laboratory (PhotoBIO-OIL), National Institute of AIST

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Abstract

We have developed a compact disc (CD)-shaped microfluidic device for multiple, rapid enzyme-linked immunosorbent assays (ELISA). The device has a versatile design that can be adapted for the detection of various proteins by selecting the push-in-type reaction parts and appropriate reagents for each target. In this paper, we report the rapid quantification of insulin, adiponectin, and leptin, which can be used for the early diagnosis of diabetes, in human serum in only 16 min with our device.

Journal

  • Analytical Sciences

    Analytical Sciences 34 (3), 379-382, 2018

    The Japan Society for Analytical Chemistry

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